RRC ID 64787
Author Kuriki M, Sato F, Arai HN, Sogabe M, Kaneko M, Kiyonari H, Kawakami K, Yoshimoto Y, Shukunami C, Sehara-Fujisawa A.
Title Transient and lineage-restricted requirement of Ebf3 for sternum ossification.
Journal Development
Abstract Osteoblasts arise from bone-surrounding connective tissue containing tenocytes and fibroblasts. Lineages of these cell populations and mechanisms of their differentiation are not well understood. Screening enhancer-trap lines of zebrafish allowed us to identify Ebf3 as a transcription factor marking tenocytes and connective tissue cells in skeletal muscle of embryos. Knockout of Ebf3 in mice had no effect on chondrogenesis but led to sternum ossification defects as a result of defective generation of Runx2+ pre-osteoblasts. Conditional and temporal Ebf3 knockout mice revealed requirements of Ebf3 in the lateral plate mesenchyme cells (LPMs), especially in tendon/muscle connective tissue cells, and a stage-specific Ebf3 requirement at embryonic day 9.5-10.5. Upregulated expression of connective tissue markers, such as Egr1/2 and Osr1, increased number of Islet1+ mesenchyme cells, and downregulation of gene expression of the Runx2 regulator Shox2 in Ebf3-deleted thoracic LPMs suggest crucial roles of Ebf3 in the onset of lateral plate mesoderm differentiation towards osteoblasts forming sternum tissues.
Volume 147(9)
Published 2020-5-12
DOI 10.1242/dev.186239
PII 147/9/dev186239
PMID 32398354
PMC PMC7240299
MeSH Animals Basic Helix-Loop-Helix Transcription Factors / metabolism Core Binding Factor Alpha 1 Subunit / metabolism Embryo, Nonmammalian / metabolism Female Fibroblasts / metabolism In Situ Hybridization LIM-Homeodomain Proteins / metabolism Mice Mice, Knockout Osteoblasts / metabolism Pregnancy RNA-Seq Sternum / metabolism Transcription Factors / genetics Transcription Factors / metabolism* Zebrafish Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 5.611
Resource
Zebrafish SAGFF(LF)19A