| RRC ID |
64820
|
| 著者 |
Sarker B, Li W, Zheng K, Detsch R, Boccaccini AR.
|
| タイトル |
Designing Porous Bone Tissue Engineering Scaffolds with Enhanced Mechanical Properties from Composite Hydrogels Composed of Modified Alginate, Gelatin, and Bioactive Glass.
|
| ジャーナル |
ACS Biomater Sci Eng
|
| Abstract |
The combination of biodegradable polymers and bioactive inorganic materials is being widely used for designing bone tissue engineering scaffolds. Here we report a composite hydrogel system composed of bioactive glass incorporated in covalently cross-linked oxidized alginate-gelatin hydrogel (ADA-GEL) for designing porous scaffolds with tunable stiffness and degradability using freeze-drying technique. Because of the presence of bioactive glass, the cross-linking kinetic and cross-linking degree of the hydrogels are significantly increased, which is the main factor for the measured enhanced mechanical strength of the bioactive glass containing ADA-GEL scaffolds. The hydrogels with high cross-linking degree exhibit low protein release profile and low degradability. Apatite formation on bioactive glass containing hydrogel-based scaffolds is confirmed by FTIR. Bone marrow-derived stromal cell growth is promoted in pristine ADA-GEL and 1% bioactive glass containing ADA-GEL scaffolds compared to the scaffolds of pure alginate, alginate-gelatin blended hydrogel, and 5% bioactive glass containing ADA-GEL. Initial studies indicated that the scaffolds, especially without bioactive glass, support osteogenic differentiation of murine bone marrow stromal cell line in the absence of foreign osteogenic stimulating supplements; however, they exhibit low levels of osteogenic expression.
|
| 巻・号 |
2(12)
|
| ページ |
2240-2254
|
| 公開日 |
2016-12-12
|
| DOI |
10.1021/acsbiomaterials.6b00470
|
| PMID |
33465897
|
| IF |
4.152
|
| リソース情報 |
| ヒト・動物細胞 |
ST2(RCB0224) |
