RRC ID 6540
Author Kiuchi T, Ohashi K, Kurita S, Mizuno K.
Title Cofilin promotes stimulus-induced lamellipodium formation by generating an abundant supply of actin monomers.
Journal J. Cell Biol.
Abstract Cofilin stimulates actin filament disassembly and accelerates actin filament turnover. Cofilin is also involved in stimulus-induced actin filament assembly during lamellipodium formation. However, it is not clear whether this occurs by replenishing the actin monomer pool, through filament disassembly, or by creating free barbed ends, through its severing activity. Using photoactivatable Dronpa-actin, we show that cofilin is involved in producing more than half of all cytoplasmic actin monomers and that the rate of actin monomer incorporation into the tip of the lamellipodium is dependent on the size of this actin monomer pool. Finally, in cofilin-depleted cells, stimulus-induced actin monomer incorporation at the cell periphery is attenuated, but the incorporation of microinjected actin monomers is not. We propose that cofilin contributes to stimulus-induced actin filament assembly and lamellipodium extension by supplying an abundant pool of cytoplasmic actin monomers.
Volume 177(3)
Pages 465-76
Published 2007-5-7
DOI 10.1083/jcb.200610005
PII jcb.200610005
PMID 17470633
PMC PMC2064820
MeSH Actin Cytoskeleton / metabolism* Actin Depolymerizing Factors / genetics* Actin Depolymerizing Factors / metabolism* Actins / metabolism* Animals COS Cells Cercopithecus aethiops Cytoplasm / metabolism* Humans Mice Pseudopodia / metabolism*
IF 8.891
Times Cited 115
Human and Animal Cells