RRC ID 66133
著者 Shigenori Inagaki, Masakazu Agetsuma, Shinya Ohara, Toshio Iijima, Tetsuichi Wazawa, Yoshiyuki Arai, Takeharu Nagai
タイトル In vivo brain activity imaging of interactively locomoting mice
ジャーナル bioRxiv
Abstract Electrophysiological field potential dynamics have been widely used to investigate brain functions and related psychiatric disorders. Conversely, however, various technical limitations of conventional recording methods have limited its applicability to freely moving subjects, especially when they are in a group and socially interacting with each other. Here, we propose a new method to overcome these technical limitations by introducing a bioluminescent voltage indicator called LOTUS-V. Using our simple and fiber-free recording method, named “SNIPA,” we succeeded in capturing brain activity in freely-locomotive mice, without the need for complicated instruments. This novel method further allowed us to simultaneously record from multiple independently-locomotive animals that were interacting with one another. Further, we successfully demonstrated that the primary visual cortex was activated during the interaction. This methodology will further facilitate a wide range of studies in neurobiology and psychiatry.
ページ October 15, 2017
公開日 2017-1-1
DOI 10.1101/203422
リソース情報
ヒト・動物細胞 293T(RCB2202)