| RRC ID |
6712
|
| 著者 |
Sakuragi N, Ogasawara N, Tanesaka E, Yoshida M.
|
| タイトル |
Functional analysis of a novel gene, DD3-3, from Dictyostelium discoideum.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
A novel gene, DD3-3, from Dictyostelium discoideum has been isolated by an mRNA differential display between a wild-type strain AX2 and a mutant HG794 which is defective in O-glycosylation. Functional analysis of the novel gene, DD3-3, was conducted by preparing a knockout mutant, DD3-3KO, and a GST:DD3-3 fusion protein. The mutant DD3-3KO cells were allowed to develop about 1.5 h earlier than the wild-type strain AX2 cells. Northern blotting analysis of the knockout mutant cells showed a remarkable downregulation of Reg A, cAMP-dependent phosphodiesterase, and overexpression of protein tyrosine kinase (PTK) during early development and its shutdown during late development. The relationship between O-glycosylation and phosphorylation involving Reg A gene is discussed.
|
| 巻・号 |
331(4)
|
| ページ |
1201-6
|
| 公開日 |
2005-6-17
|
| DOI |
10.1016/j.bbrc.2005.03.244
|
| PII |
S0006-291X(05)00734-5
|
| PMID |
15883003
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Blotting, Western
DNA, Complementary
Dictyostelium / genetics*
Genes, Protozoan*
Molecular Sequence Data
Protozoan Proteins / genetics*
RNA, Messenger / genetics
Sequence Homology, Amino Acid
|
| IF |
2.985
|
| 引用数 |
3
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 細胞性粘菌 |
G20075
G24685
G20358 |
