| RRC ID |
67568
|
| 著者 |
Zhang S, Zhao J, Lv X, Fan J, Lu Y, Zeng T, Wu H, Chen L, Zhao Y.
|
| タイトル |
Analysis on gene modular network reveals morphogen-directed development robustness in Drosophila.
|
| ジャーナル |
Cell Discov
|
| Abstract |
Genetic robustness is an important characteristic to tolerate genetic or nongenetic perturbations and ensure phenotypic stability. Morphogens, a type of evolutionarily conserved diffusible molecules, govern tissue patterns in a direction-dependent or concentration-dependent manner by differentially regulating downstream gene expression. However, whether the morphogen-directed gene regulatory network possesses genetic robustness remains elusive. In the present study, we collected 4217 morphogen-responsive genes along A-P axis of Drosophila wing discs from the RNA-seq data, and clustered them into 12 modules. By applying mathematical model to the measured data, we constructed a gene modular network (GMN) to decipher the module regulatory interactions and robustness in morphogen-directed development. The computational analyses on asymptotical dynamics of this GMN demonstrated that this morphogen-directed GMN is robust to tolerate a majority of genetic perturbations, which has been further validated by biological experiments. Furthermore, besides the genetic alterations, we further demonstrated that this morphogen-directed GMN can well tolerate nongenetic perturbations (Hh production changes) via computational analyses and experimental validation. Therefore, these findings clearly indicate that the morphogen-directed GMN is robust in response to perturbations and is important for Drosophila to ensure the proper tissue patterning in wing disc.
|
| 巻・号 |
6
|
| ページ |
43
|
| 公開日 |
2020-1-1
|
| DOI |
10.1038/s41421-020-0173-z
|
| PII |
173
|
| PMID |
32637151
|
| PMC |
PMC7324402
|
| IF |
6.255
|
| リソース情報 |
| ショウジョウバエ |
|
