| RRC ID |
67580
|
| 著者 |
Watanabe S, Hayashi R, Sasamoto Y, Tsujikawa M, Ksander BR, Frank MH, Quantock AJ, Frank NY, Nishida K.
|
| タイトル |
Human iPS cells engender corneal epithelial stem cells with holoclone-forming capabilities.
|
| ジャーナル |
iScience
|
| Abstract |
Human induced pluripotent stem cells (hiPSCs) can generate a multiplicity of organoids, yet no compelling evidence currently exists as to whether or not these contain tissue-specific, holoclone-forming stem cells. Here, we show that a subpopulation of cells in a hiPSC-derived corneal epithelial cell sheet is positive for ABCB5 (ATP-binding cassette, sub-family B, member 5), a functional marker of adult corneal epithelial stem cells. These cells possess remarkable holoclone-forming capabilities, which can be suppressed by an antibody-mediated ABCB5 blockade. The cell sheets are generated from ABCB5+ hiPSCs that first emerge in 2D eye-like organoids around six weeks of differentiation and display corneal epithelial immunostaining characteristics and gene expression patterns, including sustained expression of ABCB5. The findings highlight the translational potential of ABCB5-enriched, hiPSC-derived corneal epithelial cell sheets to recover vision in stem cell-deficient human eyes and represent the first report of holoclone-forming stem cells being directly identified in an hiPSC-derived organoid.
|
| 巻・号 |
24(6)
|
| ページ |
102688
|
| 公開日 |
2021-6-25
|
| DOI |
10.1016/j.isci.2021.102688
|
| PII |
S2589-0042(21)00656-8
|
| PMID |
34195566
|
| PMC |
PMC8233200
|
| IF |
4.447
|
| リソース情報 |
| ヒト・動物細胞 |
201B7(HPS0063) |
