| RRC ID |
6761
|
| 著者 |
Abe T, Ishikawa T, Masuda T, Mizusawa K, Tsukamoto T, Mitani H, Yanagisawa T, Todo T, Iigo M.
|
| タイトル |
Molecular analysis of Dec1 and Dec2 in the peripheral circadian clock of zebrafish photosensitive cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
To elucidate the roles of DEC1 and DEC2, basic helix-loop-helix transcription factors, in the circadian clock of photosensitive zebrafish peripheral cells, zebrafish Dec1 and Dec2 (zDec1 and zDec2) were cloned and their functions and expression patterns were examined in BRF41, a zebrafish cell line. zDEC1 and zDEC2 have high sequence similarity to mammalian counterparts and the molecular phylogenetic analysis of the zDEC1 and zDEC2 sequences reflected the predicted pattern of species classification. zDEC1 and zDEC2 inhibited zCLOCK1:zBMAL3 mediated transcription as CRY1a. zDec1 and zDec2 mRNA showed robust circadian oscillation in BRF41 cells. However, zDec1 and zDec2 mRNA was not strongly induced by exposure to light. These results indicate that zDec1 and zDec2 are involved in the circadian clock mechanism in photosensitive zebrafish peripheral cells by suppressing CLOCK/BMAL-induced gene expression and that the feedback loops of zDEC1 and zDEC2 may be interlocked with the PER/CRY core circadian feedback loops.
|
| 巻・号 |
351(4)
|
| ページ |
1072-7
|
| 公開日 |
2006-12-29
|
| DOI |
10.1016/j.bbrc.2006.10.172
|
| PII |
S0006-291X(06)02439-9
|
| PMID |
17097613
|
| MeSH |
Animals
Basic Helix-Loop-Helix Transcription Factors / genetics
Basic Helix-Loop-Helix Transcription Factors / physiology*
CLOCK Proteins
Circadian Rhythm / genetics*
Circadian Rhythm / radiation effects
Cloning, Molecular
Gene Expression Regulation*
Light
Phylogeny
Suppression, Genetic
Trans-Activators / genetics
Transcription, Genetic / radiation effects
Zebrafish / genetics
Zebrafish / physiology*
Zebrafish Proteins / genetics
Zebrafish Proteins / physiology*
|
| IF |
2.985
|
| 引用数 |
9
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
