RRC ID 67984
著者 K. Takada, N. Ohno, T. Yadomae
タイトル Physicochemical and biological characterizations of complex formation between lipopolysaccharide and lysozyme
ジャーナル Journal of Endotoxin Research
Abstract Endotoxin (lipopolysaccharide, LPS) from Gram-negative bacteria induces a variety of biological effects in mammals. We have earlier demonstrated that lysozyme (LZM) will bind to both smooth and rough forms of LPS and lipid A with high affinity, and inhibits several of the immunomodulatory activities of LPS. The production of LZM/LPS complexes was suggested to be significantly influenced by both reaction temperature and ionic strength. In this manuscript, we describe experiments designed to examine the formation of LZM/LPS complexes prepared under various conditions as assessed by fluorescence measurements of dansylated LZM and also by separation of complexes via nitration methodologies. The results of these studies provide additional experimental evidence that complex formation requires both ionic and hydrophobic interactions. The most stable complexes were formed at conditions of low ionic strength and higher temperature. Under these conditions, hydrophobic interactions were predominant in complex formation. At lower temperatures, the extent of complex formation was less, and ionic interactions were predominant. TNF production by RAW264.7 cells in vitro was decreased by LZM/LPS complexes prepared in both high and low ionic strength, but was most significantly decreased at conditions of low ionic strength and higher temperatures. These facts suggest that both ionic and hydrophobic interactions of LZM with LPS are important for neutralizing the biological activity of LPS.
巻・号 2
ページ 255-262
公開日 1995-8-1
DOI 10.1177/096805199500200405
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)