RRC ID 68499
Author Miyake M, Inufusa H, Adachi M, Ishida H, Hashida H, Tokuhara T, Kakehi Y.
Title Suppression of pulmonary metastasis using adenovirally motility related protein-1 (MRP-1/CD9) gene delivery.
Journal Oncogene
Abstract Previously we showed that MRP-1/CD9 might prevent tumor metastasis by suppression of cell motility and invasion of tissue barriers. The present study explored the possibility of preventing metastasis of mouse melanoma BL6 by expression of MRP-1/CD9 through gene transfer. A replication-deficient adenovirus vector was used for the in vivo transfer of MRP-1/CD9 cDNA. Intratumor injection of an adenovirus vector (rAd-MRP-1/CD9) expressing MRP-1/CD9 resulted in a 73.7% reduction in the number of pulmonary metastases of mice and the median survival time of mice treated with rAd-MRP-1/CD9 was significantly longer than those treated with the rAd-beta-gal vector (103.2 approximately plus;8.5 days vs 71.2 approximately plus;5.2 days, P<0.001 respectively). These results support the expression of MRP-1/CD9 through gene transfer as a therapeutic strategy for preventing metastases and prolonging survival, and support the feasibility of gene transfer in a clinically relevant setting.
Volume 19(46)
Pages 5221-6
Published 2000-11-2
DOI 10.1038/sj.onc.1203919
PMID 11077438
MeSH Adenoviridae / genetics* Adenoviridae / physiology Animals Antigens, CD / genetics Antigens, CD / physiology* Blotting, Western Cell Division Cell Movement Extremities Gene Transfer Techniques* Genetic Therapy* Immunohistochemistry Lung Neoplasms / genetics Lung Neoplasms / pathology Lung Neoplasms / secondary* Lung Neoplasms / therapy Melanoma / genetics Melanoma / pathology* Melanoma / therapy Membrane Glycoproteins* Mice Mice, Inbred BALB C Neoplasm Invasiveness Neoplasm Transplantation Survival Rate Tetraspanin 29 Transfection Transgenes / genetics Tumor Cells, Cultured
IF 7.971
Human and Animal Cells BL6