| RRC ID |
68499
|
| 著者 |
Miyake M, Inufusa H, Adachi M, Ishida H, Hashida H, Tokuhara T, Kakehi Y.
|
| タイトル |
Suppression of pulmonary metastasis using adenovirally motility related protein-1 (MRP-1/CD9) gene delivery.
|
| ジャーナル |
Oncogene
|
| Abstract |
Previously we showed that MRP-1/CD9 might prevent tumor metastasis by suppression of cell motility and invasion of tissue barriers. The present study explored the possibility of preventing metastasis of mouse melanoma BL6 by expression of MRP-1/CD9 through gene transfer. A replication-deficient adenovirus vector was used for the in vivo transfer of MRP-1/CD9 cDNA. Intratumor injection of an adenovirus vector (rAd-MRP-1/CD9) expressing MRP-1/CD9 resulted in a 73.7% reduction in the number of pulmonary metastases of mice and the median survival time of mice treated with rAd-MRP-1/CD9 was significantly longer than those treated with the rAd-beta-gal vector (103.2 approximately plus;8.5 days vs 71.2 approximately plus;5.2 days, P<0.001 respectively). These results support the expression of MRP-1/CD9 through gene transfer as a therapeutic strategy for preventing metastases and prolonging survival, and support the feasibility of gene transfer in a clinically relevant setting.
|
| 巻・号 |
19(46)
|
| ページ |
5221-6
|
| 公開日 |
2000-11-2
|
| DOI |
10.1038/sj.onc.1203919
|
| PMID |
11077438
|
| MeSH |
Adenoviridae / genetics*
Adenoviridae / physiology
Animals
Antigens, CD / genetics
Antigens, CD / physiology*
Blotting, Western
Cell Division
Cell Movement
Extremities
Gene Transfer Techniques*
Genetic Therapy*
Immunohistochemistry
Lung Neoplasms / genetics
Lung Neoplasms / pathology
Lung Neoplasms / secondary*
Lung Neoplasms / therapy
Melanoma / genetics
Melanoma / pathology*
Melanoma / therapy
Membrane Glycoproteins*
Mice
Mice, Inbred BALB C
Neoplasm Invasiveness
Neoplasm Transplantation
Survival Rate
Tetraspanin 29
Transfection
Transgenes / genetics
Tumor Cells, Cultured
|
| IF |
7.971
|
| リソース情報 |
| ヒト・動物細胞 |
BL6 |
