RRC ID 68565
著者 Herbst SA, Stolarczyk M, Becirovic T, Czernilofsky F, Liu Y, Kolb C, Knoll M, Herling M, Müller-Tidow C, Dietrich S.
タイトル Phagocytosis by stroma confounds coculture studies.
ジャーナル iScience
Abstract Signals provided by the microenvironment can modify and circumvent pathway activities that are therapeutically targeted by drugs. Bone marrow stromal cell coculture models are frequently used to study the influence of the bone marrow niche on ex vivo drug response. Here, we show that mesenchymal stromal cells from selected donors and NKTert, a stromal cell line, which is commonly used for coculture studies with primary leukemia cells, extensively phagocytose apoptotic cells. This could lead to misinterpretation of results, especially if viability readouts of the target cells (e.g. leukemic cells) in such coculture models are based on the relative proportions of dead and alive cells. Future coculture studies which aim to investigate the impact of bone marrow stromal cells on drug response should take into account that stromal cells have the capacity to phagocytose apoptotic cells.
巻・号 24(9)
ページ 103062
公開日 2021-9-24
DOI 10.1016/j.isci.2021.103062
PII S2589-0042(21)01030-0
PMID 34585113
PMC PMC8456054
IF 4.447
リソース情報
ヒト・動物細胞 StromaNKtert(RCB2350)