RRC ID 70718
著者 Wendler F, Park S, Hill C, Galasso A, Chang KR, Awan I, Sudarikova Y, Bustamante-Sequeiros M, Liu S, Sung EY, Aisa-Bonoko G, Kim SK, Baena-Lopez LA.
タイトル A LexAop > UAS > QUAS trimeric plasmid to generate inducible and interconvertible Drosophila overexpression transgenes.
ジャーナル Sci Rep
Abstract The existence of three independent binary systems for conditional gene expression (Gal4/UAS; LexA/LexAop; QF/QUAS) has greatly expanded versatile genetic analyses in the Drosophila melanogaster; however, the experimental application of these tools is limited by the need to generate multiple collections of noninterchangeable transgenic fly strains for each inducible gene expression system. To address this practical limitation, we developed a modular vector that contains the regulatory elements from all three binary systems, enabling Gal4-, LexA- or QF-dependent expression of transgenes. Our methods also incorporate DNA elements that facilitate independent site-specific recombination and elimination of regulatory UAS, LexAop or QUAS modules with spatial and temporal control, thus offering unprecedented possibilities and logistical advantages for in vivo genetic modulation and efficient interconversion of overexpression transgenic fly lines.
巻・号 12(1)
ページ 3835
公開日 2022-3-9
DOI 10.1038/s41598-022-07852-7
PII 10.1038/s41598-022-07852-7
PMID 35264662
PMC PMC8907290
MeSH Animals Animals, Genetically Modified Drosophila* / genetics Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Plasmids / genetics Transgenes
IF 3.998
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