RRC ID 71778
Author Takayuki Hoshino, Tomohiro Konno, Kazuhiko Ishihara, Keisuke Morishima
Title Cell Self Assembly of Intracellular Interface Using Cell Migration
Journal MRS Proceedings
Abstract A cell-driven self-assembly of intracellular nano-device was proposed for bio-hybrid interface. This cells-driven self-assembly employed cell migration force to insert a conductive nanoneedle which would be worked as intracellular electrode. Such a nanoneedle was fabricated in the bottom of a microwell using focused ion beam induce deposition. The microwell structure with a coating of cell adhesion molecules was employed as the scaffold of the cell migration. A glass plate with the microwells had a non cell binding coating of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer as an anti-biofouling material. Thus a cell adhered only on the wall of a microwell then the cell migrated into the microwell. Adhesion force and migration force induced self-insertion of the nanoneedle into a live cell body using the cell's own migration force. The inserted nanoneedle was made of electrical conductive tungsten, so the intracellular nanoneedle might extract intracellular potential more precisely than extracellular electrode, while inducing much less damage to cells. In the future, the technique of cell-driven self-insertion of nanoneedle may be integrated with multi electrode arrays for developing long-lasting measurements device on cellular network researches, or the risk assessment of the nanomaterials on cellular activities.
Volume 1092
Published 2008-1-1
DOI 10.1557/proc-1092-bb02-12
Human and Animal Cells C2C12(RCB0987)