| RRC ID |
72275
|
| 著者 |
Uchida N, Drysdale CM, Nassehi T, Gamer J, Yapundich M, DiNicola J, Shibata Y, Hinds M, Gudmundsdottir B, Haro-Mora JJ, Demirci S, Tisdale JF.
|
| タイトル |
Cas9 protein delivery non-integrating lentiviral vectors for gene correction in sickle cell disease.
|
| ジャーナル |
Mol Ther Methods Clin Dev
|
| Abstract |
Gene editing with the CRISPR-Cas9 system could revolutionize hematopoietic stem cell (HSC)-targeted gene therapy for hereditary diseases, including sickle cell disease (SCD). Conventional delivery of editing tools by electroporation limits HSC fitness due to its toxicity; therefore, efficient and non-toxic delivery remains crucial. Integrating lentiviral vectors are established for therapeutic gene delivery to engraftable HSCs in gene therapy trials; however, their sustained expression and size limitation preclude their use for CRISPR-Cas9 delivery. Here, we developed a Cas9 protein delivery non-integrating lentiviral system encoding guide RNA and donor DNA, allowing for transient endonuclease function and inclusion of all editing tools in a single vector (all-in-one). We demonstrated efficient one-time correction of the SCD mutation in the endogenous βs-globin gene up to 42% at the protein level (p < 0.01) with the Cas9 protein delivery non-integrating lentiviral all-in-one system without electroporation. Our findings improve prospects for efficient and safe genome editing.
|
| 巻・号 |
21
|
| ページ |
121-132
|
| 公開日 |
2021-6-11
|
| DOI |
10.1016/j.omtm.2021.02.022
|
| PII |
S2329-0501(21)00037-1
|
| PMID |
33816645
|
| PMC |
PMC8005818
|
| IF |
4.533
|
| リソース情報 |
| ヒト・動物細胞 |
HUDEP-2(RCB4457) |
