RRC ID 73004
著者 Yamanaka R, Zullo SA, Tanaka R, Blaese M, Xanthopoulos KG.
タイトル Enhancement of antitumor immune response in glioma models in mice by genetically modified dendritic cells pulsed with Semliki forest virus-mediated complementary DNA.
ジャーナル J Neurosurg
Abstract OBJECT:The aim of this study was to further investigate dendritic cell (DC)-based immunotherapy for malignant glioma to improve its therapeutic efficacy.
METHODS:Dendritic cells were isolated from the bone marrow and pulsed with phosphate-buffered saline, tumor RNA, tumor lysate, Semliki Forest virus (SFV)-LacZ, SFV-mediated B16 complementary (c)DNA, or SFV-mediated 203 glioma cDNA, respectively, to treat mice bearing tumors of the 203 glioma cell line. The results indicated that pre-immunization with DCs pulsed with the same type of cDNA as in the tumor by a self-replicating RNA vector (that is, SFV) protected mice from tumor challenge, and that therapeutic immunization prolonged the survival of mice with established tumors. The SFV induced apoptosis in DCs and their death facilitated the uptake of apoptotic cells by other DCs, thus providing a potential mechanism for enhanced immunogenicity.
CONCLUSIONS:Therapy with DCs that have been pulsed with SFV-mediated tumor cDNA may be an excellent procedure for the development of new cancer vaccines.
巻・号 94(3)
ページ 474-81
公開日 2001-3-1
DOI 10.3171/jns.2001.94.3.0474
PMID 11235953
MeSH Animals Apoptosis / genetics Apoptosis / immunology Bone Marrow Cells / cytology Brain Neoplasms / mortality Brain Neoplasms / therapy* CD8-Positive T-Lymphocytes / immunology DNA, Complementary Dendritic Cells / cytology Dendritic Cells / immunology* Genetic Therapy / methods* Genetic Vectors* Glioma / mortality Glioma / therapy* Immunization Immunotherapy / methods* Melanoma Mice Mice, Inbred C57BL Semliki forest virus* Survival Rate Transfection Tumor Cells, Cultured
IF 3.968
リソース情報
ヒト・動物細胞 ST2(RCB0224)