RRC ID 73115
著者 Jayendran Srinivasan, Vincent Kish, Sydha Salihu, Madhavi Ayyalasomayajula, Nilay Mukherjee
タイトル Poking Cells in Cell-Gel Constructs: A Potential Way of Measuring Fluid Pressure in Cells
ジャーナル Advances in Bioengineering
Abstract In this study, fluorescently labeled ATDC5 and bone marrow derived preosteoblastic cells were embedded in agarose gel and poked with a micropipette under a confocal microscope. Image stacks of cross sections of cells were taken before and after poking and the largest cross sectional areas were analyzed. After adjusting for photobleaching effects by equalizing the area of a nearby unpoked cell (by adjusting threshold values of the images) the area of the poked cell before and after poking was measured and the percentage change in area was calculated. The percentage change in cross sectional area was 25 ± 15% for ATDC5 cells and 31 ±13% for bone marrow derived cells (n= 5 for each type of cell). The decrease in area was statistically significant for each cell type (p<0.05). There was no difference in the reduction in areas between the two types of cells (power >80%). These results are consistent with the theory that some of the cell water is free and some are structured or restricted. This method can be utilized to determine fluid pressure in cells. This is significant because it is believed that changes in intracellular fluid pressure can play a role in cellular mechanotransduction.
公開日 2004-1-1
DOI 10.1115/imece2004-61290
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)