RRC ID 73116
著者 Madhavi Ayyalasomayajula, Sydha Salihu, Vincent Kish, Nilay Mukherjee
タイトル Influence of Rearrangement of Actin Cytoskeleton on the Overall Material Properties of ATDC5 Cells During Chondrogenesis
ジャーナル Advances in Bioengineering
Abstract ATDC5 cells, a prechondrocytic cell line, were cultured in 2% agarose gel-cell constructs. A major reorientation of actin structure (stress-fiber to punctate) was observed during the chondrogenesis of these cells. 2D finite element models of cell (considered as a composite with embedded actin fibers) in a 500μ3 gel were created for days 1 (prechondrocytes), 5, 8 & 12 (chondrocytes) of culture. The response of the cells to uniaxial compression of the gel-cell construct was studied. The model predicted that, as a result of rearrangement of actin, the cells became more compliant and less anisotropic. When the gel size was reduced to 20μ3, RUTL (Ratio of radial deformations of cell in the Transverse direction to Loaded direction) became significantly different between prechondrocytes and chondrocytes. The difference in force required to compress a 20μ3 gel-cell construct containing a prechondrocyte vs a chondrocyte to 15% deformation was 103nN (152.3 vs 48.8 nN). This information can be used to build MEMS based devices that can mechanically distinguish between the different cell types.
公開日 2004-1-1
DOI 10.1115/imece2004-61286
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)