| RRC ID |
73741
|
| 著者 |
Onishi K, Ishihara S, Takahashi M, Sakai A, Enomoto A, Suzuki K, Haga H.
|
| タイトル |
Substrate stiffness induces nuclear localization of myosin regulatory light chain to suppress apoptosis.
|
| ジャーナル |
FEBS Lett
|
| Abstract |
Stiffness of the extracellular matrix regulates various biological responses, but the response mechanisms are poorly understood. Here, we found that the nuclear diphosphorylated myosin regulatory light chain (2P-MRLC) is a critical mechanomediator that suppresses apoptosis in response to substrate stiffness. Stiff substrates promoted the nuclear localization of 2P-MRLC. Zipper-interacting protein kinase [ZIPK; also known as death-associated protein kinase 3 (DAPK3)], a kinase for MRLC, was localized in the nucleus in response to stiff substrates and promoted the nuclear localization of 2P-MRLC. Moreover, actin fiber formation induced by substrate stiffness promoted the nuclear localization of 2P-MRLC via ZIPK. 2P-MRLC in response to substrate stiffness suppressed the expression of MAF bZIP transcription factor B (MafB) and repressed apoptosis. These findings reveal a newly identified role of MRLC in mechanotransduction.
|
| 公開日 |
2023-2-1
|
| DOI |
10.1002/1873-3468.14592
|
| PMID |
36723402
|
| IF |
3.057
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007) |
