| RRC ID |
74476
|
| 著者 |
Ebata H, Umeda K, Nishizawa K, Nagao W, Inokuchi S, Sugino Y, Miyamoto T, Mizuno D.
|
| タイトル |
Activity-dependent glassy cell mechanics Ⅰ: Mechanical properties measured with active microrheology.
|
| ジャーナル |
Biophys J
|
| Abstract |
Active microrheology was conducted in living cells by applying an optical-trapping force to vigorously-fluctuating tracer beads with feedback-tracking technology. The complex shear modulus G(ω)=G'(ω)-iG″(ω) was measured in HeLa cells in an epithelial-like confluent monolayer. We found that G(ω)∝(-iω)1/2 over a wide range of frequencies (1 Hz <ω/2π <10 kHz). Actin disruption and cell-cycle progression from G1 to S and G2 phases only had a limited effect on G(ω) in living cells. On the other hand, G(ω) was found to be dependent on cell metabolism; ATP-depleted cells showed an increased elastic modulus G'(ω) at low frequencies, giving rise to a constant plateau such that G(ω)=G0+A(-iω)1/2 . Both the plateau and the additional frequency dependency ∝(-iω)1/2 of ATP-depleted cells are consistent with a rheological response typical of colloidal jamming. On the other hand, the plateau G0 disappeared in ordinary metabolically active cells, implying that living cells fluidize their internal states such that they approach the critical jamming point.
|
| 公開日 |
2023-4-11
|
| DOI |
10.1016/j.bpj.2023.04.011
|
| PII |
S0006-3495(23)00241-2
|
| PMID |
37050875
|
| IF |
3.854
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007)
HeLa/Fucci2(RCB2867) |
