| RRC ID |
75634
|
| 著者 |
Takahashi T, Shirai J, Matsuda M, Nakanaga S, Matsushita S, Wakita K, Hayashishita M, Suzuki R, Noguchi A, Yokota N, Kawahara H.
|
| タイトル |
Protein quality control machinery supports primary ciliogenesis by eliminating GDP-bound Rab8-family GTPases.
|
| ジャーナル |
iScience
|
| Abstract |
The small GTPase Rab8 plays a vital role in the vesicular trafficking of cargo proteins from the trans-Golgi network to target membranes. Upon reaching its target destination, Rab8 is released from the vesicular membrane into the cytoplasm via guanosine triphosphate (GTP) hydrolysis. The fate of GDP-bound Rab8 released from the destination membranes, however, has not been investigated adequately. In this study, we found that GDP-bound Rab8 subfamily proteins are targeted for immediate degradation, and the pre-emptive quality control machinery is responsible for eliminating these proteins in a nucleotide-specific manner. We provide evidence that components of this quality control machinery have a critical role in vesicular trafficking events, including the formation of primary cilia, a process regulated by the Rab8 subfamily. These results suggest that the protein degradation machinery plays a critical role in the integrity of membrane trafficking by limiting the excessive accumulation of GDP-bound Rab8 subfamily proteins.
|
| 巻・号 |
26(5)
|
| ページ |
106652
|
| 公開日 |
2023-5-19
|
| DOI |
10.1016/j.isci.2023.106652
|
| PII |
S2589-0042(23)00729-0
|
| PMID |
37182096
|
| PMC |
PMC10173616
|
| IF |
4.447
|
| リソース情報 |
| ヒト・動物細胞 |
293T(RCB2202) |
