RRC ID 77215
Author Hirotoshi Yasui, Kazuomi Takahashi, Shunichi Taki, Misae Shimizu, Chiaki Koike, Koji Umeda, Shofiqur Rahman, Tomohiro Akashi, Van Sa Nguyen, Yoshiyuki Nakagawa, Kazuhide Sato
Title Near Infrared Photo‐Antimicrobial Targeting Therapy for Candida albicans
Journal Advanced Therapeutics
Abstract Drug‐resistant microorganisms are a pressing issue, and innovative antimicrobial therapies are required; antibodies targeting antigens on pathogen surfaces have emerged as one such modality. IgY, abundant in chicken egg yolk, confers passive immunity, and is effective against various pathogens; however, its antimicrobial effects remain limited. Near‐infrared photoimmunotherapy (NIR‐PIT), originally developed as a cancer treatment, specifically kills cancer cells via a photosensitizing phthalocyanine dye (IRDye700Dx; IR700)‐conjugated monoclonal antibody, and irradiating NIR light. IgY‐photo‐antimicrobial targeting therapy (IgY‐PAT2), exploiting NIR‐PIT, is investigated to destroy only microorganisms. IR700 is conjugated with anti‐Candida albicans IgY (CA‐IgY) to generate CA‐IgY‐IR700, which specifically binds various Candida spp. (and not human skin cells). The antimicrobial effect of CA‐IgY‐PAT2 is dependent on the NIR‐light dose (p < 0.0001). CA‐IgY‐PAT2 significantly reduces the area of ulcers in a mouse model of CA‐infected cutaneous ulcers (p < 0.0001), indicating that CA‐IgY‐PAT2 is a new promising therapeutic method for CA infection.
Volume 4
Published 2021-2-1
DOI 10.1002/adtp.202000221
General Microbes JCM 1542