| RRC ID |
77570
|
| Author |
Katada Y, Kunimi H, Serizawa N, Lee D, Kobayashi K, Negishi K, Okano H, Tanaka KF, Tsubota K, Kurihara T.
|
| Title |
Starburst amacrine cells amplify optogenetic visual restoration through gap junctions.
|
| Journal |
Mol Ther Methods Clin Dev
|
| Abstract |
Ectopic induction of optogenetic actuators, such as channelrhodopsin, is a promising approach to restoring vision in the degenerating retina. However, the cell type-specific response of ectopic photoreception has not been well understood. There are limits to obtaining efficient gene expression in a specifically targeted cell population by a transgenic approach. In the present study, we established a murine model with high efficiency of gene induction to retinal ganglion cells (RGCs) and amacrine cells using an improved tetracycline transactivator-operator bipartite system (KENGE-tet system). To investigate the cell type-specific visual restorative effect, we expressed the channelrhodopsin gene into RGCs and amacrine cells using the KENGE-tet system. As a result, enhancement in the visual restorative effect was observed to RGCs and starburst amacrine cells. In conclusion, a photoresponse from amacrine cells may enhance the maintained response of RGCs and further increase or improve the visual restorative effect.
|
| Volume |
30
|
| Pages |
1-13
|
| Published |
2023-9-14
|
| DOI |
10.1016/j.omtm.2023.05.011
|
| PII |
S2329-0501(23)00073-6
|
| PMID |
37324975
|
| PMC |
PMC10265492
|
| IF |
4.533
|
| Resource |
| Mice |
RBRC05445
RBRC09550
RBRC09551
RBRC05843 |
