RRC ID 77570
Author Katada Y, Kunimi H, Serizawa N, Lee D, Kobayashi K, Negishi K, Okano H, Tanaka KF, Tsubota K, Kurihara T.
Title Starburst amacrine cells amplify optogenetic visual restoration through gap junctions.
Journal Mol Ther Methods Clin Dev
Abstract Ectopic induction of optogenetic actuators, such as channelrhodopsin, is a promising approach to restoring vision in the degenerating retina. However, the cell type-specific response of ectopic photoreception has not been well understood. There are limits to obtaining efficient gene expression in a specifically targeted cell population by a transgenic approach. In the present study, we established a murine model with high efficiency of gene induction to retinal ganglion cells (RGCs) and amacrine cells using an improved tetracycline transactivator-operator bipartite system (KENGE-tet system). To investigate the cell type-specific visual restorative effect, we expressed the channelrhodopsin gene into RGCs and amacrine cells using the KENGE-tet system. As a result, enhancement in the visual restorative effect was observed to RGCs and starburst amacrine cells. In conclusion, a photoresponse from amacrine cells may enhance the maintained response of RGCs and further increase or improve the visual restorative effect.
Volume 30
Pages 1-13
Published 2023-9-14
DOI 10.1016/j.omtm.2023.05.011
PII S2329-0501(23)00073-6
PMID 37324975
PMC PMC10265492
IF 4.533
Mice RBRC05445 RBRC09550 RBRC09551 RBRC05843