Reference - Detail
RRC ID | 81657 |
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Author | Kurata A, Aimatsu K, Kimura Y, Hashiguchi H, Maeda A, Imai T, Yamasaki-Yashiki S, Hamada K, Fujimoto Y, Fujii A, Uegaki K. |
Title | Characterization of the membrane vesicle fraction from Acetobacter sp. WSS15. |
Journal | J Biosci Bioeng |
Abstract |
A bacterium that produces membrane vesicles (MVs), strain WSS15, was isolated from a traditional vinegar in Japan called Kurozu. A phylogenetic analysis of 16S rRNA gene sequences indicated that this bacterium belongs to the genus Acetobacter. MVs and peptidoglycan-associated lipoprotein (Pal) were detected in the MV fraction of strain WSS15. In the presence of the WSS15 MV fraction, murine macrophages produced the pro-inflammatory cytokine interleukin-6 (IL-6) via the recognition by superficial Toll-like receptor 2 (TLR2). WSS15 MVs adhered to the cell surface of macrophages. The macrophages secreted IL-6 through the TLR2 recognition of an acylated N-terminal peptide of Pal. We elucidated the mode of action of WSS15 MVs on immune cells and identified the Pal peptide from strain WSS15 as an agonist of TLR2. |
Published | 2024-9-5 |
DOI | 10.1016/j.jbiosc.2024.07.017 |
PII | S1389-1723(24)00228-7 |
PMID | 39242325 |
Resource | |
Human and Animal Cells | RAW 264(RCB0535) |