RRC ID 81657
Author Kurata A, Aimatsu K, Kimura Y, Hashiguchi H, Maeda A, Imai T, Yamasaki-Yashiki S, Hamada K, Fujimoto Y, Fujii A, Uegaki K.
Title Characterization of the membrane vesicle fraction from Acetobacter sp. WSS15.
Journal J Biosci Bioeng
Abstract A bacterium that produces membrane vesicles (MVs), strain WSS15, was isolated from a traditional vinegar in Japan called Kurozu. A phylogenetic analysis of 16S rRNA gene sequences indicated that this bacterium belongs to the genus Acetobacter. MVs and peptidoglycan-associated lipoprotein (Pal) were detected in the MV fraction of strain WSS15. In the presence of the WSS15 MV fraction, murine macrophages produced the pro-inflammatory cytokine interleukin-6 (IL-6) via the recognition by superficial Toll-like receptor 2 (TLR2). WSS15 MVs adhered to the cell surface of macrophages. The macrophages secreted IL-6 through the TLR2 recognition of an acylated N-terminal peptide of Pal. We elucidated the mode of action of WSS15 MVs on immune cells and identified the Pal peptide from strain WSS15 as an agonist of TLR2.
Published 2024-9-5
DOI 10.1016/j.jbiosc.2024.07.017
PII S1389-1723(24)00228-7
PMID 39242325
Resource
Human and Animal Cells RAW 264(RCB0535)