| RRC ID |
82093
|
| Author |
Ujie Y, Saito S, Fukaya K, Urabe D, Yaguchi T, Arai MA.
|
| Title |
Aspergillus terreus IFM 65899-THP-1 cells interaction triggers production of the natural product butyrolactone Ia, an immune suppressive compound.
|
| Journal |
Sci Rep
|
| Abstract |
We focused on the possibility that pathogenic microorganisms might produce immune suppressors to evade the action of immune cells. Based on this possibility, we have recently developed new co-culture method of pathogenic actinomyces and immune cells, however, the interaction mechanism between pathogens and cells was still unclear. In this report, co-culturing pathogenic fungi and immune cells were investigated. Pathogenic fungus Aspergillus terreus IFM 65899 and THP-1 cells were co-cultured and isolated a co-culture specific compound, butyrolactone Ia (1). 1 inhibits the production of nitric oxide by RAW264 cells and exhibits regulatory effects on autophagy, suggesting 1 plays a defensive role in the response of A. terreus IFM 65899 to immune cells. Furthermore, dialysis experiments and micrographs indicated that "physical interaction" between A. terreus IFM 65899 and THP-1 cells may be required for the production of 1. This is the first report of co-culture method of fungi with immune cells and its interaction mechanism.
|
| Volume |
14(1)
|
| Pages |
28278
|
| Published |
2024-11-16
|
| DOI |
10.1038/s41598-024-79837-7
|
| PII |
10.1038/s41598-024-79837-7
|
| PMID |
39550448
|
| PMC |
PMC11569209
|
| MeSH |
4-Butyrolactone* / analogs & derivatives
4-Butyrolactone* / metabolism
Animals
Aspergillus* / metabolism
Autophagy
Biological Products / pharmacology
Coculture Techniques*
Humans
Mice
Nitric Oxide* / metabolism
RAW 264.7 Cells
THP-1 Cells
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
