| RRC ID |
85362
|
| Author |
Zhu Y, Cai P, Li Z, Zhang S, Kong WT, Wang H, Gao F, Zeng Y, Qian J, Su B, Liu Z, Ginhoux F.
|
| Title |
Transcription factors TCF4 and KLF4 respectively control the development of the DC2A and DC2B lineages.
|
| Journal |
Nat Immunol
|
| Abstract |
Conventional dendritic cells (cDCs) are a heterogeneous population of professional antigen-presenting cells that bridge innate and adaptive immunity. Many studies in mice have identified various populations of cDCs whose inter-relationships and discrete identities, as well as their link to plasmacytoid DCs (pDCs), have not been cohesively addressed. Here, by combining single-cell sequencing, transcription factor fate-mapping models, conditional knockout models and adoptive transfer, we show that Klf4 expression clearly separates cDC lineage from the pDC lineage, and defined two pre-DC2 subsets: Siglec-H+CD115- pre-DC2s and Siglec-HloCD115+ pre-DC2s. While Siglec-H+CD115- pre-DC2s represent the pDC-like cells that give rise to CD7+CD11blo DC2As in a TCF4-dependent manner, Siglec-HloCD115+ pre-DC2s give rise to CD7-CD11bhi DC2Bs in a KLF4-dependent manner. These data reveal the transcriptional basis of two pre-DC2 subsets and present a firm framework for mouse cDC classification, paving the way for a better understanding of these cells in tissues and in disease.
|
| Published |
2025-7-23
|
| DOI |
10.1038/s41590-025-02208-5
|
| PII |
10.1038/s41590-025-02208-5
|
| PMID |
40702338
|
| IF |
20.479
|
| Resource |
| Mice |
RBRC02704 |
