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論文 - 一覧

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  • 検索条件 : 絞込み (MeSH = Cloning, Molecular / methods*)
生物種 リソース名 タイトル
ショウジョウバエ Systematic identification of genes regulating synaptic remodeling in the Drosophila visual system.
細胞性粘菌 AX4 A GoldenBraid cloning system for synthetic biology in social amoebae.
ヒト・動物細胞 F-36P(RCB0775) A transmembrane trap method for efficient cloning of genes encoding proteins possessing transmembrane domain.
原核生物(枯草菌) MBS794 , MBS795 , MBS796 , MBS797 , MBS798 , MBS799 , MBS800 , MBS801 , MBS802 , MBS803 , ... Conjugational transfer system to shuttle giant DNA cloned by Bacillus subtilis genome (BGM) vector.
原核生物(枯草菌) MBS902 , MBS903 , MBS904 , MBS905 , MBS906 , MBS907 , MBS908 , MBS909 , MBS910 , MBS911 , ... Combining two genomes in one cell: stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome.
一般微生物 JCM20190 Chaperone-assisted expression and purification of putrescine monooxygenase from Shewanella putrefaciens-95.
一般微生物 JCM20779 Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination.
遺伝子材料 pCRT (RDB17479) , CRZeroT (RDB17480) , pCRZero (RDB17481) A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products.
一般微生物 JCM 18229 Efficient construction of xenogeneic genomic libraries by circumventing restriction-modification systems that restrict methylated DNA.
遺伝子材料 Lambda GEX5 (RDB01911) MNK1, a new MAP kinase-activated protein kinase, isolated by a novel expression screening method for identifying protein kinase substrates.
遺伝子材料 pAP3neo cDNA Library Mouse 10T-half (RDB02903) Large scale isolation of osteoclast-specific genes by an improved method involving the preparation of a subtracted cDNA library.
遺伝子材料 mouse SDF-5 cDNA (RDB06782) , mouse SDR-1 alpha cDNA (RDB06783) , mouse SDR-1 beta cDNA (RDB06784). Characterization of novel secreted and membrane proteins isolated by the signal sequence trap method.
遺伝子材料 mouse SDF-1 alpha cDNA (RDB06778) , mouse SDF-1 beta cDNA (RDB06779). Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins.
遺伝子材料 pDUAL-HFF1 (RDB06179) , pDUAL-HFF41 (RDB06180) , pDUAL-HFF81 (RDB06181). Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts.
ヒト・動物細胞 MRC-5 Encapsulation of cells in agarose beads for use in the construction of human DNA libraries as yeast artificial chromosomes (YAC).
カイコ Genome engineering and parthenocloning in the silkworm, Bombyx mori.
遺伝子材料 RII-pcDNA1.1Amp (RDB02853) , Smad4-pcDNA1.1Amp (RDB02854). Screening cloned PCR fragments by restriction endonuclease finger-printing to obtain wild-type sequences.
酵母 BYP6848(pNHK12) , BYP6743(pNHK36) , BYP6744(pNHK53) Gateway vectors for efficient artificial gene assembly in vitro and expression in yeast Saccharomyces cerevisiae.
原核生物(大腸菌) pGBNKBOR , pNKBOR NKBOR, a mini-Tn10-based transposon for random insertion in the chromosome of Gram-negative bacteria and the rapid recovery of sequences flanking the insertion sites in Escherichia coli.
原核生物(大腸菌) pTSA30 , pTSC30 , pTSK30 New cloning vectors with temperature-sensitive replication.