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  • Search Condition : Filter (MeSH = Cloning, Molecular / methods*)
Species Resource
Arabidopsis / Cultured plant cells, genes pda05545 , pda03530 , pda01560 , pda04389 , pda04574 Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments.
Arabidopsis / Cultured plant cells, genes pda07078 , pda08126 High-throughput protein expression using a combination of ligation-independent cloning (LIC) and infrared fluorescent protein (IFP) detection.
Yeast ? A polymerase chain reaction-mediated yeast artificial chromosome-splitting technology for generating targeted yeast artificial chromosomes subclones.
Yeast BY5679(SH5679) , BY964(SH964) , BY5210(SH5210) , BY1875(SH1875) , BY1994(SH1994) , BY5481(SH5481) , BY1388(SH1388) , BY6173(SH6173) A yeast artificial chromosome-splitting vector designed for precise manipulation of specific plant chromosome region.
Yeast BY6173(SH6173) , BY5679(SH5679) A versatile and general splitting technology for generating targeted YAC subclones.
General Microbes JCM 9820 The terminal 5' phosphate and proximate phosphorothioate promote ligation-independent cloning.
Medaka full length cDNA (olecno10_i02 , olecno3_i19 , olebno16_g13 , olebrno16_g02 , olebno62_l03) High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins.
Yeast BY2778 , BY21467 High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins.
Medaka O. hubbsi (RS273) , O. javanicus(Penang) (RS831) , O. celebensis , O. curvinotus , O. dancena , O. luzonensis (RS270) , O. marmoratus (RS280) , O. mekongensis , O. minutillus Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the W chromosomes of medaka fishes.
DNA material Human HLA-A*02:01:01 cDNA (RDB02866) , Human HLA-A*02:06 cDNA (RDB02867) , Human HLA-A*02:07 cDNA (RDB02868) , Human HLA-A*11:01:01 cDNA (RDB02869) , Human HLA-A*26:01 cDNA (RDB02870) , Human HLA-A*24:02:01 cDNA (RDB02871) , Human HLA-A*31:01:02 cDNA (RDB02872) , Human HLA-A*33:03 cDNA (RDB02873) , Human HLA-B*07:02:01 cDNA (RDB02874) , Human HLA-B*15:01:01 cDNA (RDB02875) , ... Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines.
Cellular slime molds G90010 Green fluorescent protein and epitope tag fusion vectors for Dictyostelium discoideum.
DNA material Lambda GEX5 (RDB01911) MNK1, a new MAP kinase-activated protein kinase, isolated by a novel expression screening method for identifying protein kinase substrates.
Prokaryotes E. coli pSL775 Carotenoid-biosynthesis genes as a genetic marker for the purpose of gene cloning.
Prokaryotes E. coli pKLJ10 , pKLJ12 Construction and characterization of F plasmid-based expression vectors.
Prokaryotes E. coli pHC79 A small cosmid for efficient cloning of large DNA fragments.
Prokaryotes E. coli placZ1 , placZ1a , placZ2 , placZ3 , placZY1 , placZY1a , placZY2 , placZY3 New improved lacZ gene fusion vectors.
Prokaryotes E. coli pCW107 A versatile and highly repressible Escherichia coli expression system based on invertible promoters: expression of a gene encoding a toxic product.
Prokaryotes E. coli pCBC2 , pCBC1 New vectors for direct cloning of PCR products.
Prokaryotes E. coli pKF2 , pKF3 Construction and characterization of new host-vector systems for the enforcement-cloning method.
Prokaryotes E. coli pETGEXCT A T7 expression vector for producing N- and C-terminal fusion proteins with glutathione S-transferase.