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  • Search Condition : Filter (MeSH = Gene Targeting / methods*)
Species Resource Title
Chicken / Quail Primordial germ cell-specific expression of eGFP in transgenic chickens.
Medaka TG1403 olvas-DsRedExpress Generation of biallelic F0 mutants in medaka using the CRISPR/Cas9 system.
Human and Animal Cells CHO-K1(RCB0285) Targeted transgene insertion into the CHO cell genome using Cre recombinase-incorporating integrase-defective retroviral vectors.
Mice RBRC01515 , RBRC02975 Optimizing Nervous System-Specific Gene Targeting with Cre Driver Lines: Prevalence of Germline Recombination and Influencing Factors.
DNA material YCX2.60/pCS2 (RDB15455) Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.
Drosophila CAS-0002 An undergraduate laboratory class using CRISPR/Cas9 technology to mutate drosophila genes.
Medaka d-rR/TOKYO (MT837) , OK-Cab (MT830) , DJ-1(delta7) (MT972) , MC4R(delta7) (MT973) , MC4R(delta11) (MT974) , Sakyo (WS1164) Design, evaluation, and screening methods for efficient targeted mutagenesis with transcription activator-like effector nucleases in medaka.
Human and Animal Cells LK-2(RCB1970) Discovery of a dicer-independent, cell-type dependent alternate targeting sequence generator: implications in gene silencing & pooled RNAi screens.
Human and Animal Cells Wi-38 Transcriptional targeting of adenovirus vectors with the squamous cell carcinoma-specific antigen-2 promoter for selective apoptosis induction in lung cancer.
DNA material pHIS3K (RDB06561) , pLYS1K (RDB06553) , pARG1K (RDB06557) , pHIS3U (RDB06560) , pLYS1U (RDB06552) , pARG1U (RDB06556) , pHIS3H (RDB06562) , pLYS1H (RDB06554) , pARG1H (RDB06558) , pHIS3B (RDB06563) , ... A novel series of vectors for chromosomal integration in fission yeast.
Silkworms CRISPR/Cas9-mediated knockout of factors in non-homologous end joining pathway enhances gene targeting in silkworm cells.
DNA material AAVS1 T2 CRIPR in pX330 (RDB13917) , pMK232 (AAVS1 CMV-OsTIR1-PURO) (RDB13918) , pMK243 (TetOne OsTIR1 AAVS1) (RDB13919) , pMK277 (mClover-NeoR) (RDB13920) , pMK278 (mClover-Hygro) (RDB13921) , pMK279 (mClover-Bsr) (RDB13922) , pMK280 (mCherry2-NeoR) (RDB13923) , pMK281 (mCherry2-Hygro) (RDB13924) , pMK282 (mCherry2-Bsr) (RDB13925) , pMK283 (Stag-3FLAG-Neo) (RDB13926) , ... Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.
DNA material px330-Tyr-M (RDB13943) , pCAG-EGxxFP-Tyr-M (RDB13944). Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system.
Yeast BY28260 , BY28261 , BY28262 , BY28263 , BY28264 , BY28265 , BY28266 , BY28267 , BY28268 , BY28269 , ... iAID: an improved auxin-inducible degron system for the construction of a 'tight' conditional mutant in the budding yeast Saccharomyces cerevisiae.
DNA material pCAG-EGxxFP (RDB13532) , pX330-Cetn1/sgRNA#1 (RDB13533) , pX330-Prm1/sgRNA#1 (RDB13534). Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.
Rats , Human and Animal Cells F344/Stm(strainID=266) , Rat-1(RCB1830) Simple knockout by electroporation of engineered endonucleases into intact rat embryos.
Cellular slime molds G90002 , G90003 A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system.
Medaka database Nanos3 gene targeting in medaka ES cells.
Medaka database Parameters and efficiency of direct gene disruption by zinc finger nucleases in medaka embryos.
Prokaryotes E. coli ME5076 Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.