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  • Search Condition : Filter (MeSH = Gene Targeting / methods*)
Species Resource
INFORMATION E.coli Gene targeting using randomly inserted group II introns (targetrons) recovered from an Escherichia coli gene disruption library.
Yeast BY8229(AH22R-) Nanoparticles for the delivery of genes and drugs to human hepatocytes.
Prokaryotes E. coli CV-25(DH5alpha) , pINT-ts , pAH69 , pAH121 , pAH130 Replicon-free and markerless methods for genomic insertion of DNAs in phage attachment sites and controlled expression of chromosomal genes in Escherichia coli.
Human embryonic stem cells KhES-1 , KhES-3 Highly efficient transient gene expression and gene targeting in primate embryonic stem cells with helper-dependent adenoviral vectors.
Rats F344/NSlc(strainID=379) , ACI/NSlc(strainID=381) , BN/SsNSlc(strainID=371) An ENU-induced mutant archive for gene targeting in rats.
Mice C57BL/6-Tg(CAG-FLPe)37Ito/ItoRbrc Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
DNA material pBluescript SKII+AV1-235-4491 (RDB01505) Adeno-associated virus Rep-mediated targeting of integrase-defective retroviral vector DNA circles into human chromosome 19.
Zebrafish Review Transposon-mediated stable integration and tetracycline-inducible expression of electroporated transgenes in chicken embryos.
Silkworms NA Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection.
Medaka Tg(olvas-GFP) (TG870) , d-rR/TOKYO (MT837) , Database Targeted disruption of exogenous EGFP gene in medaka using zinc-finger nucleases.
Prokaryotes E. coli ME5076 Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.
Medaka database Parameters and efficiency of direct gene disruption by zinc finger nucleases in medaka embryos.
Medaka database Nanos3 gene targeting in medaka ES cells.
Cellular slime molds G90002 , G90003 A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system.
Rats F344/Stm(strainID=266) Simple knockout by electroporation of engineered endonucleases into intact rat embryos.
DNA material px330-Tyr-M (RDB13943) , pCAG-EGxxFP-Tyr-M (RDB13944). Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system.
Silkworms CRISPR/Cas9-mediated knockout of factors in non-homologous end joining pathway enhances gene targeting in silkworm cells.
DNA material YCX2.60/pCS2 (RDB15455) Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.
Drosophila CAS-0002 An undergraduate laboratory class using CRISPR/Cas9 technology to mutate drosophila genes.
DNA material pHIS3K (RDB06561) , pLYS1K (RDB06553) , pARG1K (RDB06557) , pHIS3U (RDB06560) , pLYS1U (RDB06552) , pARG1U (RDB06556) , pHIS3H (RDB06562) , pLYS1H (RDB06554) , pARG1H (RDB06558) , pHIS3B (RDB06563) , ... A novel series of vectors for chromosomal integration in fission yeast.