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  • Search Condition : Filter (MeSH = Genetic Engineering / methods*)
Species Resource Title
DNA material AxCBCas9 (RDB19290) , AxCBNC9 (RDB19291) Construction of adenovirus vectors simultaneously expressing four multiplex, double-nicking guide RNAs of CRISPR/Cas9 and in vivo genome editing.
Human and Animal Cells CHO-K1(RCB0285) An attempt to add biological functions by genetic engineering in order to produce high-performance bioreactor cells for hybrid artificial liver: transfection of glutamine synthetase into Chinese hamster ovary (CHO) cell.
Human and Animal Cells CMES40(AES0166) Efficient marmoset genome engineering by autologous embryo transfer and CRISPR/Cas9 technology.
Human and Animal Cells CHO-K1(RCB0285) Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids.
Human and Animal Cells HUDEP-2(RCB4557) CRISPR-SURF: discovering regulatory elements by deconvolution of CRISPR tiling screen data.
Human and Animal Cells A549(RCB0098) Synergistic Upregulation of Target Genes by TET1 and VP64 in the dCas9-SunTag Platform.
DNA material pUC18K_ePAG2 (RDB17947) , pUC18Z_ePAG2 (RDB17948) Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli.
DNA material pCRT (RDB17479) , CRZeroT (RDB17480) , pCRZero (RDB17481) A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products.
Algae NIES-144 Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis.
Prokaryotes E. coli Cloning Vector pJB658 Markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida.
Prokaryotes E. coli BW25113, ΔyggS, Δggt Production of Ophthalmic Acid Using Engineered Escherichia coli.
Prokaryotes E. coli W3110 Markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida.
DNA material , Human and Animal Cells pCMV-VSV-G-RSV-Rev (RDB04393) , pCAG-HIVgp (RDB04394) , 201B7(HPS0063) Establishment of a reporter system to monitor silencing status in induced pluripotent stem cell lines.
DNA material , Human and Animal Cells B6N Mouse BAC clone B6Ng01-263  (RDB07573) , 10T1/2(RCB0247) A method of producing genetically manipulated mouse mammary gland.
Prokaryotes E. coli Using the CRISPR-Cas System to Positively Select Mutants in Genes Essential for Its Function.
Arabidopsis / Cultured plant cells, genes pda10875 Photoactivatable CRISPR-Cas9 for optogenetic genome editing.
Human and Animal Cells C2C12(RCB0987) , HeLa Hypoxia-responsive transgene expression system using RTP801 promoter and synthetic transactivator fused with oxygen-dependent degradation domain.
Human and Animal Cells B16/BL6(RCB2638) Exosome-based tumor antigens-adjuvant co-delivery utilizing genetically engineered tumor cell-derived exosomes with immunostimulatory CpG DNA.
Human and Animal Cells 293T(RCB2202) Development of Genome Engineering Tools from Plant-Specific PPR Proteins Using Animal Cultured Cells.
DNA material T7-NLS hCas9-pA (RDB13130). CLICK: one-step generation of conditional knockout mice.