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  • Search Condition : Filter (MeSH = Genetic Engineering / methods*)
Species Resource
Human and Animal Cells HUDEP-2(RCB4557) CRISPR-SURF: discovering regulatory elements by deconvolution of CRISPR tiling screen data.
DNA material pUC18K_ePAG2 (RDB17947) , pUC18Z_ePAG2 (RDB17948) Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli.
DNA material pCRT (RDB17479) , CRZeroT (RDB17480) , pCRZero (RDB17481) A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products.
Algae NIES-144 Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis.
Prokaryotes E. coli Cloning Vector pJB658 Markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida.
Prokaryotes E. coli BW25113, ΔyggS, Δggt Production of Ophthalmic Acid Using Engineered Escherichia coli.
Prokaryotes E. coli W3110 Markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida.
DNA material , Human and Animal Cells pCMV-VSV-G-RSV-Rev (RDB04393) , pCAG-HIVgp (RDB04394) , 201B7(HPS0063) Establishment of a reporter system to monitor silencing status in induced pluripotent stem cell lines.
DNA material , Human and Animal Cells B6N Mouse BAC clone B6Ng01-263  (RDB07573) , 10T1/2(RCB0247) A method of producing genetically manipulated mouse mammary gland.
Rats W-Tg(CAG-cre)81Jmsk (StrainID=534) CLICK: one-step generation of conditional knockout mice.
Prokaryotes E. coli Using the CRISPR-Cas System to Positively Select Mutants in Genes Essential for Its Function.
Arabidopsis / Cultured plant cells, genes pda10875 Photoactivatable CRISPR-Cas9 for optogenetic genome editing.
Human and Animal Cells C2C12(RCB0987) Hypoxia-responsive transgene expression system using RTP801 promoter and synthetic transactivator fused with oxygen-dependent degradation domain.
Human and Animal Cells B16/BL6(RCB2638) Exosome-based tumor antigens-adjuvant co-delivery utilizing genetically engineered tumor cell-derived exosomes with immunostimulatory CpG DNA.
Human and Animal Cells 293T(RCB2202) Development of Genome Engineering Tools from Plant-Specific PPR Proteins Using Animal Cultured Cells.
DNA material T7-NLS hCas9-pA (RDB13130). CLICK: one-step generation of conditional knockout mice.
Prokaryotes E. coli Production of Ophthalmic Acid Using Engineered Escherichia coli.
Mice B6.Cg-Tg(CAG-Cre)CZ-MO2Osb(RBRC01828) CLICK: one-step generation of conditional knockout mice.
Drosophila D. ananassae white mutant Transgenic tools for members of the genus Drosophila with sequenced genomes.
Tomato TOMJPF00001 Improvement of the transient expression system for production of recombinant proteins in plants.