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  • Search Condition : Filter (MeSH = Mutagenesis, Insertional / methods*)
Species Resource
INFORMATION E.coli High-throughput transposon mutagenesis of Corynebacterium glutamicum and construction of a single-gene disruptant mutant library.
Prokaryotes E. coli CV-25(DH5alpha) , pINT-ts , pAH69 , pAH121 , pAH130 Replicon-free and markerless methods for genomic insertion of DNAs in phage attachment sites and controlled expression of chromosomal genes in Escherichia coli.
Zebrafish XIG8A , SAGp22A , HG6D? , HG21D? Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafish.
Zebrafish ? Analysis of genes and genome by the tol2-mediated gene and enhancer trap methods.
General Microbes JCM 3201 New vector system for random, single-step integration of multiple copies of DNA into the Rhodococcus genome.
Yeast ? A set of loxP marker cassettes for Cre-mediated multiple gene disruption in Schizosaccharomyces pombe.
Cellular slime molds G90116 Highly effective removal of floxed Blasticidin S resistance cassettes from Dictyostelium discoideum mutants by extrachromosomal expression of Cre.
Lotus / Glycine LORE1 Establishment of a Lotus japonicus gene tagging population using the exon-targeting endogenous retrotransposon LORE1.
Prokaryotes E. coli pJP5603 , pJP5608 An improved suicide vector for construction of chromosomal insertion mutations in bacteria.
Prokaryotes E. coli pAB1001 , pAB1002 , pAB2001 , pAB2002 , pMS246 , pMS252 , pMS255 , pMS266 , pMS272 New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions.
Prokaryotes E. coli pMR-7wt Construction and expression of a modular gene encoding bacteriophage T7 RNA polymerase.
Prokaryotes E. coli Transposon insertion disruptant A novel, simple, high-throughput method for isolation of genome-wide transposon insertion mutants of Escherichia coli K-12.
Zebrafish SAGp53A , SAGm18B Transposon-mediated gene trapping in zebrafish.
Cellular slime molds A novel gene trap method using terminator-REMI and 3' rapid amplification of cDNA ends (RACE) in Dictyostelium.
DNA material pxCANCre (RDB1675) An accumulative site-specific gene integration system using Cre recombinase-mediated cassette exchange.
General Microbes JCM 1170 A convenient method for multiple insertions of desired genes into target loci on the Escherichia coli chromosome.
Prokaryotes E. coli JW2669-KC Silent mischief: bacteriophage Mu insertions contaminate products of Escherichia coli random mutagenesis performed using suicidal transposon delivery plasmids mobilized by broad-host-range RP4 conjugative machinery.
DNA material pUPAT-egfp3ic (RDB07884) , pUPAT-SRV (RDB07885) Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells.
Cellular slime molds S00155 Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA.
Human and Animal Cells CHO-K1 Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems.