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  • 検索条件 : 絞込み (MeSH = Mutagenesis, Insertional / methods*)
生物種 リソース名 タイトル
酵母 BYP5041 , BYP5042 New plasmids for the disruption and repeated use of selection markers in Saccharomyces cerevisiae.
原核生物(枯草菌) MBS959 , MBS960 , MBS961 , MBS962 , MBS963 , MBS964 , MBS965 , MBS966 , MBS967 , MBS968 , ... Genetic transfer of large DNA inserts to designated loci of the Bacillus subtilis 168 genome.
ヒト・動物細胞 CHO-K1 Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems.
原核生物(大腸菌) Transposon insertion disruptant A novel, simple, high-throughput method for isolation of genome-wide transposon insertion mutants of Escherichia coli K-12.
原核生物(大腸菌) JW2669-KC Silent mischief: bacteriophage Mu insertions contaminate products of Escherichia coli random mutagenesis performed using suicidal transposon delivery plasmids mobilized by broad-host-range RP4 conjugative machinery.
一般微生物 JCM 1170 A convenient method for multiple insertions of desired genes into target loci on the Escherichia coli chromosome.
原核生物(大腸菌) pMR-7wt Construction and expression of a modular gene encoding bacteriophage T7 RNA polymerase.
原核生物(大腸菌) pAB1001 , pAB1002 , pAB2001 , pAB2002 , pMS246 , pMS252 , pMS255 , pMS266 , pMS272 New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions.
原核生物(大腸菌) pJP5603 , pJP5608 An improved suicide vector for construction of chromosomal insertion mutations in bacteria.
ショウジョウバエ DGRC#109052 Trap a gene and find out its function: toward functional genomics in Drosophila.
ミヤコグサ・ダイズ LORE1 Establishment of a Lotus japonicus gene tagging population using the exon-targeting endogenous retrotransposon LORE1.
細胞性粘菌 G90116 Highly effective removal of floxed Blasticidin S resistance cassettes from Dictyostelium discoideum mutants by extrachromosomal expression of Cre.
細胞性粘菌 S00155 Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA.
遺伝子材料 pUPAT-egfp3ic (RDB07884) , pUPAT-SRV (RDB07885) Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells.
遺伝子材料 pxCANCre (RDB1675) An accumulative site-specific gene integration system using Cre recombinase-mediated cassette exchange.
細胞性粘菌 A novel gene trap method using terminator-REMI and 3' rapid amplification of cDNA ends (RACE) in Dictyostelium.
一般微生物 JCM 3201 New vector system for random, single-step integration of multiple copies of DNA into the Rhodococcus genome.
ゼブラフィッシュ Analysis of genes and genome by the tol2-mediated gene and enhancer trap methods.
原核生物(大腸菌) CV-25(DH5alpha) , pINT-ts , pAH69 , pAH121 , pAH130 Replicon-free and markerless methods for genomic insertion of DNAs in phage attachment sites and controlled expression of chromosomal genes in Escherichia coli.
ゼブラフィッシュ Fuji28, Fuji70, gSAG37B, XIG8A, SAGp22A, HG6D, HG21B Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafish.