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  • Search Condition : Filter (MeSH = Protein Engineering / methods*)
Species Resource Title
DNA material , Human and Animal Cells pGEM-scFv-T7-A36 (RDB19420) , pGEM-scFv-GFPA36 (RDB19421) , pET-scFv-GFPA36 (RDB19422) , pIRES-scFv-GFPA36 (RDB19423) , pGEM-scFv-A36 mutant (M4) (RDB19424) , pGEM-scFv-GFPA36 mutant (M4) (RDB19425) , pGEM-s3Flag-scFv-A36 (RDB19426) , pGEM-s3Flag-scFv-M4 (RDB19427) , ER-scFv-A36-expressing vector (RDB19428) , 293T(RCB2202) , ... An ultra-stable cytoplasmic antibody engineered for in vivo applications.
DNA material , Human and Animal Cells AAV:NT-probe-nuc-mCherry (RDB19166) , AAV:CT-probe-cyto-mCherry (RDB19167) , 293T(RCB2202) Diffusible GRAPHIC to visualize morphology of cells after specific cell-cell contact.
Human and Animal Cells Jurkat Lasso-grafting of macrocyclic peptide pharmacophores yields multi-functional proteins.
Human and Animal Cells HeLa Design Strategy to Create Antibody Mimetics Harbouring Immobilised Complementarity Determining Region Peptides for Practical Use.
DNA material Rat alpha-Antitryspin RRRRSL mutant (RDB03410) , Rat alpha-Antitrypsin RRRRSQ mutant (RDB03411) Engineering of alpha1-antitrypsin variants selective for subtilisin-like proprotein convertases PACE4 and PC6: importance of the P2' residue in stable complex formation of the serpin with proprotein convertase.
DNA material pCAG-EGFP-NpF2164g5_BV4 (RDB17331) Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin.
DNA material pET-TuTs (RDB17457) , pBAD33betaH (RDB17458) , pBAD33Ts-Tu-beta-3 (RDB17459) Functional Qbeta replicase genetically fusing essential subunits EF-Ts and EF-Tu with beta-subunit.
Zebrafish Tg(huC:GVP) A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters.
Human and Animal Cells Ba/F3(RCB0805) Engineering a growth sensor to select intracellular antibodies in the cytosol of mammalian cells.
Human and Animal Cells Expression of an antibody-enzyme complex by the L-chain fusion method.
Human and Animal Cells Production of recombinant tumor necrosis factor receptor/Fc fusion protein by genetically manipulated chickens.
Human and Animal Cells A549(RCB0098) Novel extracellular matrix for cell sheet recovery using genetically engineered elastin-like protein.
Human and Animal Cells RAW 264(RCB0535) Extracellular expression of a functional recombinant Ganoderma lucidium immunomodulatory protein by Bacillus subtilis and Lactococcus lactis.
Human and Animal Cells Improved production of recombinant human antithrombin III in Chinese hamster ovary cells by ATF4 overexpression.
Human and Animal Cells Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increasing point mutation.
Prokaryotes E. coli JW5702-KC , ASKA collection Improving ethanol tolerance of Escherichia coli by rewiring its global regulator cAMP receptor protein (CRP).
Prokaryotes E. coli JW0715-KC , ASKA clone Assignment of function to histidines 260 and 298 by engineering the E1 component of the Escherichia coli 2-oxoglutarate dehydrogenase complex; substitutions that lead to acceptance of substrates lacking the 5-carboxyl group.
DNA material , Human and Animal Cells pSV2 erb B2 (RDB01273) , Ba/F3(RCB0805) Ligand-inducible dimeric antibody for selecting antibodies against a membrane protein based on mammalian cell proliferation.
Silkworms d17 Production of small antibacterial peptides using silkworm-baculovirus protein expression system.
General Microbes JCM 9174 Gene cloning and characterization of dihydrolipoamide dehydrogenase from Microbacterium luteolum: A useful enzymatic regeneration system of NAD+ from NADH.