| RRC ID |
45805
|
| 著者 |
Shikata H, Yakushijin Y, Matsushita N, Sakai A, Sugita A, Nakamura N, Yamanouchi J, Azuma T, Hato T, Yasukawa M.
|
| タイトル |
Role of activation-induced cytidine deaminase in the progression of follicular lymphoma.
|
| ジャーナル |
Cancer Sci
|
| Abstract |
Activation-induced cytidine deaminase (AID/AICDA) is required for somatic hypermutation and class-switch recombination of the immunoglobulin gene, and for c-myc translocation of germinal center-derived B-cell lymphoma. In the present study, we attempted to clarify the significance of AID associated with c-myc in the progression of follicular lymphoma (FL) using RT-PCR and quantitative real-time PCR. Tissues from the patients with grade 3 FL expressed relatively higher levels of c-myc and AID. The samples taken from a patient with FL who died within 2 years after the start of treatment showed either no or low expression of AID, despite expressing high levels of c-myc. In order to examine the role of AID expression in rapidly progressive FL, the full-length AID transcript was transfected into AID-negative cell lines established from different patients with rapidly progressive FL. This led to the establishment of AID-expressing transfectants with a low proliferation rate and a significantly increased incidence of G(0)/G(1) arrest compared with controls. Our results indicate that AID may act as a negative regulator of cell survival in FL when sufficient c-myc is expressed. Switch-off or low expression of AID after c-myc amplification may correlate with the clinical outcomes of FL.
|
| 巻・号 |
103(3)
|
| ページ |
415-21
|
| 公開日 |
2012-3-1
|
| DOI |
10.1111/j.1349-7006.2011.02186.x
|
| PMID |
22168746
|
| PMC |
PMC7713616
|
| MeSH |
Adult
Aged
Blotting, Western
Cell Proliferation
Cytidine Deaminase / genetics
Cytidine Deaminase / metabolism*
Disease Progression
Enzyme Activation
Female
Gene Expression Profiling
Humans
Immunohistochemistry
Lymphoma, Follicular / genetics
Lymphoma, Follicular / metabolism*
Lymphoma, Follicular / pathology
Male
Middle Aged
Proto-Oncogene Proteins c-myc / genetics
Proto-Oncogene Proteins c-myc / metabolism*
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Transfection
|
| IF |
4.966
|
| 引用数 |
8
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| 遺伝子材料 |
CSII-CMV-MCS-IRES2-Bsd (RDB04385)
pCAG-HIVgp (RDB04394)
pCMV-VSV-G-RSV-Rev (RDB04393). |
