論文 - 詳細
| RRC ID | 18239 |
|---|---|
| 著者 | Morimura R, Komatsu S, Ichikawa D, Takeshita H, Tsujiura M, Nagata H, Konishi H, Shiozaki A, Ikoma H, Okamoto K, Ochiai T, Taniguchi H, Otsuji E. |
| タイトル | Novel diagnostic value of circulating miR-18a in plasma of patients with pancreatic cancer. |
| ジャーナル | Br J Cancer |
| Abstract |
BACKGROUND:Several recent studies have demonstrated that microRNAs (miRNAs) are stably detectable in the plasma/serum. We hypothesised that miR-18a in the plasma is a potential biomarker in patients with pancreatic cancer. METHODS:miR-18a is located in the miR-17-92 cluster and reported to be highly expressed in pancreatic cancer tissues. This study was divided into three parts: (1) Confirmation of higher miR-18a levels in primary pancreatic cancer tissues and cell lines than in normal pancreatic tissues and a human fibroblast cell line. (2) Evaluation of the plasma miR-18a assay using quantitative RT-PCR by comparing plasma results obtained from 36 patients with pancreatic cancer and from 30 healthy volunteers. (3) Evaluation of the assay for monitoring tumour dynamics in patients with pancreatic cancer. RESULTS:(1) The expression of miR-18a was significantly higher in pancreatic cancer tissues (P=0.012) and pancreatic cancer cell lines (P=0.015) than in normal tissues and fibroblasts. (2) Plasma concentrations of miR-18a were significantly higher in pancreatic cancer patients than in controls (P<0.0001). The value of the area under the receiver-operating characteristic curve (AUC) was 0.9369. (3) Plasma levels of miR-18a were significantly lower in postoperative samples than in preoperative samples (P=0.0077). CONCLUSION:Circulating miR-18a might provide new complementary tumour markers for pancreatic cancer. |
| 巻・号 | 105(11) |
| ページ | 1733-40 |
| 公開日 | 2011-11-22 |
| DOI | 10.1038/bjc.2011.453 |
| PII | bjc2011453 |
| PMID | 22045190 |
| PMC | PMC3242609 |
| MeSH | Adolescent Adult Aged Aged, 80 and over Biomarkers, Tumor / blood* Biomarkers, Tumor / genetics Cell Line, Tumor Child Child, Preschool Female Fibroblasts / metabolism Genetic Testing / methods Humans Infant Infant, Newborn Male MicroRNAs / blood* MicroRNAs / genetics Middle Aged Pancreatic Neoplasms / blood Pancreatic Neoplasms / genetics* Pancreatic Neoplasms / pathology Real-Time Polymerase Chain Reaction / methods Reverse Transcriptase Polymerase Chain Reaction / methods Young Adult |
| IF | 5.791 |
| 引用数 | 165 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | PANC-1(RCB2095) NOR-P1(RCB2139) PK-45P(RCB2141) KP4-1(RCB1006) PK-59(RCB1901) |