| RRC ID |
38255
|
| 著者 |
Cui L, Ohuchida K, Mizumoto K, Moriyama T, Onimaru M, Nakata K, Nabae T, Ueki T, Sato N, Tominaga Y, Tanaka M.
|
| タイトル |
Prospectively isolated cancer-associated CD10(+) fibroblasts have stronger interactions with CD133(+) colon cancer cells than with CD133(-) cancer cells.
|
| ジャーナル |
PLoS One
|
| Abstract |
Although CD133 has been reported to be a promising colon cancer stem cell marker, the biological functions of CD133+ colon cancer cells remain controversial. In the present study, we investigated the biological differences between CD133+ and CD133- colon cancer cells, with a particular focus on their interactions with cancer-associated fibroblasts, especially CD10+ fibroblasts. We used 19 primary colon cancer tissues, 30 primary cultures of fibroblasts derived from colon cancer tissues and 6 colon cancer cell lines. We isolated CD133+ and CD133- subpopulations from the colon cancer tissues and cultured cells. In vitro analyses revealed that the two populations showed similar biological behaviors in their proliferation and chemosensitivity. In vivo analyses revealed that CD133+ cells showed significantly greater tumor growth than CD133- cells (P=0.007). Moreover, in cocultures with primary fibroblasts derived from colon cancer tissues, CD133+ cells exhibited significantly more invasive behaviors than CD133- cells (P<0.001), especially in cocultures with CD10+ fibroblasts (P<0.0001). Further in vivo analyses revealed that CD10+ fibroblasts enhanced the tumor growth of CD133+ cells significantly more than CD10- fibroblasts (P<0.05). These data demonstrate that the in vitro invasive properties and in vivo tumor growth of CD133+ colon cancer cells are enhanced in the presence of specific cancer-associated fibroblasts, CD10+ fibroblasts, suggesting that the interactions between these specific cell populations have important roles in cancer progression. Therefore, these specific interactions may be promising targets for new colon cancer therapies.
|
| 巻・号 |
5(8)
|
| ページ |
e12121
|
| 公開日 |
2010-8-12
|
| DOI |
10.1371/journal.pone.0012121
|
| PII |
e12121
|
| PMID |
20711432
|
| PMC |
PMC2920818
|
| MeSH |
AC133 Antigen
Adult
Aged
Aged, 80 and over
Animals
Antigens, CD / metabolism*
Biomarkers, Tumor / metabolism
Cell Communication
Cell Line, Tumor
Cell Proliferation
Cell Separation
Coculture Techniques
Colonic Neoplasms / genetics
Colonic Neoplasms / pathology*
Colonic Neoplasms / surgery
Female
Fibroblasts / metabolism*
Fibroblasts / pathology*
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Glycoproteins / deficiency*
Glycoproteins / metabolism*
Humans
Male
Mice
Middle Aged
Neoplasm Invasiveness
Neoplasm Metastasis
Neprilysin / deficiency
Neprilysin / genetics
Neprilysin / metabolism*
Peptides / deficiency*
Peptides / metabolism*
Prospective Studies
Recurrence
|
| IF |
2.74
|
| 引用数 |
20
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COLO205(RCB2127) |
