論文 - 詳細
| RRC ID | 38596 |
|---|---|
| 著者 | Kushida T, Makino T, Tomomura M, Tomomura A, Sakagami H. |
| タイトル | Enhancement of Dectin-2 gene expression by lignin-carbohydrate complex from Lentinus edodes mycelia extract (LEM) in a mouse macrophage-like cell line. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND:Lignin fractions of Lentinus edodes mycelia extract (LEM) have shown anti-HIV and immunopotentiating activity. However, the action point of lignin-carbohydrate complex has not been elucidated. In order to elucidate their action point, DNA microarray analysis was performed, using mouse macrophage-like J774.1 cells. MATERIALS AND METHODS:RNA was isolated with Qiagen RNeasy Plus Mini kit, hydridized with GeneChip MouseGene 1.0 ST arrays, and scanned with Affymetrix GeneChip Command Console software. RESULTS:One of the seven lignin-carbohydrate fractions isolated from LEM (Fr4) enhanced the expression of dectin-2 (4.2-fold) and toll-like receptor (TLR)-2 (2.5-fold) prominently, but only slightly modified the expression of dectin-1 (0.8-fold), complement receptor 3 (0.9-fold), TLR1, 3, 4, 9 and 13 (0.8- to 1.7-fold), spleen tyrosine kinase (Syk)b, zeta-chain (TCR) associated 70 kDa protein kinase (Zap70), Janus tyrosine kinase (Jak)2 (1.0- to 1.2-fold), nuclear factor (Nf)кb1, NFкb2, reticuloendotheliosis viral oncogene homolog (Rel)a, Relb (1.0- to 1.6-fold), Nfкbia, Nfкbib, Nfкbie, Nfкbil2 and Nfкbiz (0.8- to 2.3-fold). On the other hand, lipopolysaccharide did not affect the expression of dectin-2 or TLR-2. CONCLUSION:These data suggest the significant role of the activation of the dectin-2 signaling pathway in the action of lignin-carbohydrate complex on macrophages. |
| 巻・号 | 31(4) |
| ページ | 1241-8 |
| 公開日 | 2011-4-1 |
| PII | 31/4/1241 |
| PMID | 21508371 |
| MeSH | Animals Biomarkers, Tumor / genetics* Biomarkers, Tumor / metabolism Carbohydrates / chemistry* Fungal Proteins / pharmacology* Gene Expression Profiling Lectins, C-Type / genetics* Lectins, C-Type / metabolism* Lignin / metabolism* Lipopolysaccharides / pharmacology Macrophages / metabolism* Mice Oligonucleotide Array Sequence Analysis Polysaccharides / pharmacology* Principal Component Analysis Tumor Necrosis Factor-alpha / genetics Tumor Necrosis Factor-alpha / metabolism |
| IF | 1.994 |
| 引用数 | 8 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | J774.1 |