RRC ID |
41792
|
著者 |
Okamura H, Morimoto H, Fujita M, Nasu F, Sasakia E, Haneji T.
|
タイトル |
Suppression of Egr-1 expression in human oral squamous carcinoma cells by okadaic acid.
|
ジャーナル |
Oral Oncol
|
Abstract |
We examined the expression of early growth response-1 (Egr-1) gene in human oral squamous carcinoma cell lines SCCKN and SCC-25 cells and human osteoblastic cell lines Saos-2 and MG63 cells treated with okadaic acid, a potent inhibitor of protein phosphatases type 1 and type 2A. Western blot analysis revealed that Egr-1 was strongly expressed in SCCKN cells and that okadaic acid decreased the expression of Egr-1 protein in these cells. However, Egr-1 was expressed at lower levels in SCC-25, Saos-2, and MG63 cells and transiently increased with the okadaic acid treatment. Suppression of Egr-1 protein expression in okadaic acid-treated SCCKN cells stemmed from the suppression of the Egr-1 mRNA level, as determined by the RT-RCR method. Formaldehyde-fixed and alcohol-permeabilized cultured SCCKN cells were reacted with the anti-Egr-1 antibody using immunohistochemical methods. Intense fluorescence was observed in the nuclei of the control SCCKN cells interacted with anti-Egr-1 antibody. However, only a weak reaction was observed in the nuclei in SCCKN cells treated with okadaic acid. A gel mobility shift assay showed that treatment of SCCKN cells with okadaic acid suppressed Egr-1 binding to the DIG-labeled Egr-1 consensus oligonucleotide probe. The present results indicate that the alteration of phosphorylation states in SCCKN cells regulates Egr-1 binding to its consensus sequence and its expression at the transcriptional level.
|
巻・号 |
38(8)
|
ページ |
779-84
|
公開日 |
2002-12-1
|
DOI |
10.1016/s1368-8375(02)00039-8
|
PII |
S1368-8375(02)00039-8
|
PMID |
12570057
|
MeSH |
Carcinogens / therapeutic use*
Carcinoma, Squamous Cell / drug therapy*
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / metabolism
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Down-Regulation
Early Growth Response Protein 1
Electrophoresis, Agar Gel / methods
Gene Expression Regulation
Humans
Immediate-Early Proteins*
Immunohistochemistry / methods
Mouth Neoplasms / drug therapy*
Mouth Neoplasms / genetics
Mouth Neoplasms / metabolism
Neoplasm Proteins / genetics
Neoplasm Proteins / metabolism*
Okadaic Acid / therapeutic use*
Reverse Transcriptase Polymerase Chain Reaction / methods
Suppression, Genetic
Transcription Factors / genetics
Transcription Factors / metabolism*
Tumor Cells, Cultured
|
IF |
3.979
|
引用数 |
7
|
WOS 分野
|
DENTISTRY, ORAL SURGERY & MEDICINE
ONCOLOGY
|
リソース情報 |
ヒト・動物細胞 |
SCCKN(RCB0441) |