| RRC ID |
42230
|
| 著者 |
Nakashima K, Shimada H, Ochiai T, Kuboshima M, Kuroiwa N, Okazumi S, Matsubara H, Nomura F, Takiguchi M, Hiwasa T.
|
| タイトル |
Serological identification of TROP2 by recombinant cDNA expression cloning using sera of patients with esophageal squamous cell carcinoma.
|
| ジャーナル |
Int J Cancer
|
| Abstract |
We applied serological analysis of recombinant cDNA expression libraries (SEREX) to cases of esophageal squamous cell carcinoma (SCC) to identify tumor antigens. One of the clones identified was TROP2, which is known as calcium signal transducer. To evaluate the clinical significance of serum anti-TROP2 antibodies (s-TROP2-Abs) in patients with esophageal SCC, the presence of s-TROP2-Abs was analyzed by Western blotting using bacterially expressed TROP2 protein. We found that 23 of 75 (31%) patients were positive for s-TROP2-Abs. Positivity in terms of s-TROP2-Abs showed a significant association with tumor size but not with other clinicopathological features. The protein expression levels of TROP2 were much higher in esophageal SCC cell lines as compared to those in normal esophageal mucosa and its immortalized cells although the mRNA expression levels were not necessarily elevated in malignant cell lines and tissues. Immunohistochemical studies showed that the expression of TROP2 protein in esophageal SCC specimens was noticeably higher than that found in mild hyperplasia of esophageal mucosae. Thus, s-TROP2-Abs seemed useful in the diagnosis of SCC and may be a candidate for serum tumor markers.
|
| 巻・号 |
112(6)
|
| ページ |
1029-35
|
| 公開日 |
2004-12-20
|
| DOI |
10.1002/ijc.20517
|
| PMID |
15386348
|
| MeSH |
Aged
Antigens, Neoplasm / analysis*
Antigens, Neoplasm / immunology
Biomarkers, Tumor / analysis*
Biomarkers, Tumor / immunology
Blotting, Western
Carcinoma, Squamous Cell / chemistry*
Carcinoma, Squamous Cell / diagnosis*
Cell Adhesion Molecules / analysis*
Cell Adhesion Molecules / immunology
Cell Line, Tumor
Cloning, Molecular / methods
DNA, Complementary / analysis
DNA, Neoplasm / analysis
Epithelial Cell Adhesion Molecule
Esophageal Neoplasms / chemistry*
Esophageal Neoplasms / diagnosis*
Female
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Male
Middle Aged
Recombinant Proteins / analysis
|
| IF |
5.145
|
| 引用数 |
60
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
EC-GI-10(RCB0774) |
