論文 - 詳細
| RRC ID | 42628 |
|---|---|
| 著者 | Kanaji S, Saito H, Tsujitani S, Matsumoto S, Tatebe S, Kondo A, Ozaki M, Ito H, Ikeguchi M. |
| タイトル | Expression of polo-like kinase 1 (PLK1) protein predicts the survival of patients with gastric carcinoma. |
| ジャーナル | Oncology |
| Abstract |
OBJECTIVE:To assess the prognostic value of polo-like kinase 1 (PLK1), an important regulator of cell cycle progression, in patients with gastric carcinoma. METHODS:PLK1 expression was determined in 160 gastric carcinoma patients by immunohistochemistry and compared with p53 expression and the proliferating cell nuclear antigen-labeling index (PCNA-LI) to evaluate the effect of PLK1 on tumor progression. Furthermore, PLK mRNA expression was determined in 26 advanced gastric cancer patients by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS:PLK mRNA expression was detected in 25 (96.2%) patients by RT-PCR. Immunohistochemical staining revealed PLK1 expression in 84 (52.5%) patients. There were no significant relationships between PLK1 expression and various clinicopathological factors. PLK1 expression was significantly correlated with the PCNA-LI, but not p53 expression. The prognosis of patients with PLK1-positive tumors was significantly worse than that of patients with PLK1-negative tumors (p < 0.05). Moreover, multivariate analysis revealed that PLK1 expression was an independent prognostic factor. Patients with PLK1-positive and high PCNA-LI tumors showed a significantly poorer prognosis than patients with PLK1-negative and/or low PCNA-LI tumors. Furthermore, the prognosis of patients with PLK1- and p53-positive tumors was significantly worse than that of patients with PLK1- and p53-negative or PLK1-negative and p53-positive tumors. CONCLUSION:PLK1 expression may be a critical indicator of a poor prognosis in patients with gastric carcinoma. |
| 巻・号 | 70(2) |
| ページ | 126-33 |
| 公開日 | 2006-1-1 |
| DOI | 10.1159/000093003 |
| PII | 93003 |
| PMID | 16645325 |
| MeSH | Biomarkers, Tumor / analysis* Biomarkers, Tumor / genetics Blotting, Western Cell Cycle Proteins / analysis* Cell Cycle Proteins / genetics Cell Line, Tumor Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Multivariate Analysis Predictive Value of Tests Prognosis Proliferating Cell Nuclear Antigen / analysis Protein Serine-Threonine Kinases / analysis* Protein Serine-Threonine Kinases / genetics Proto-Oncogene Proteins / analysis* Proto-Oncogene Proteins / genetics RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction Stomach Neoplasms / chemistry Stomach Neoplasms / mortality* Stomach Neoplasms / pathology* Survival Analysis Tumor Suppressor Protein p53 / analysis |
| IF | 2.642 |
| 引用数 | 49 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MKN1(RCB1003) MKN28(RCB1000) MKN45(RCB1001) MKN74(RCB1002) Kato III(RCB2088) |