論文 - 詳細
| RRC ID | 43934 |
|---|---|
| 著者 | Nagaya H, Tagawa M, Hiwasa K, Terao S, Kanno T, Nishizaki T, Gotoh A. |
| タイトル | Fiber-substituted conditionally replicating adenovirus for oncolysis of human renal carcinoma cells. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND:Adenovirus vectors have lately been highlighted in gene therapies. We investigated the oncolytic effects of a chimeric adenovirus type 5 (Ad5) with replacement of Ad5 fiber knob with adenovirus type 35 (Ad35) fiber knob (Ad5F35) on human renal cell carcinoma (RCC). MATERIALS AND METHODS:The conditionally replicating Ad5F35 vector was constructed and infected into RCC cell lines 786-O, ACHN, and RCC4-VHL. For these cells, reverse transcription-polymerase chain reaction and western blotting were carried out and the cell viability was assayed. RESULTS:In all RCC cell lines, it was found that CD46, a cell surface target of Ad35, was well-expressed, while coxsackie and adenovirus receptor (CAR), a cell surface target of Ad5, was considerably less expressed. The Ad5F35 vector induced oncolysis of RCC cells, with significantly higher efficacy as compared with that for the Ad5 vector. CONCLUSION:Ad5F35 vector could be a candidate for promising gene therapy of human RCC. |
| 巻・号 | 32(7) |
| ページ | 2985-9 |
| 公開日 | 2012-7-1 |
| PII | 32/7/2985 |
| PMID | 22753762 |
| MeSH | Adenoviridae / genetics Adenoviridae / physiology* Adenovirus E1 Proteins / genetics Carcinoma, Renal Cell / therapy* Carcinoma, Renal Cell / virology* Cell Line, Tumor Coxsackie and Adenovirus Receptor-Like Membrane Protein Cytokines / genetics Genetic Therapy / methods Genetic Vectors HEK293 Cells Humans Kidney Neoplasms / therapy* Kidney Neoplasms / virology* Membrane Cofactor Protein / biosynthesis Membrane Cofactor Protein / genetics Midkine Oncolytic Virotherapy / methods* Promoter Regions, Genetic RNA, Messenger / biosynthesis RNA, Messenger / genetics Receptors, Virus / biosynthesis Receptors, Virus / genetics Virus Replication |
| IF | 1.994 |
| 引用数 | 2 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |