RRC ID 45723
著者 Devanapally S, Ravikumar S, Jose AM.
タイトル Double-stranded RNA made in C. elegans neurons can enter the germline and cause transgenerational gene silencing.
ジャーナル Proc Natl Acad Sci U S A
Abstract An animal that can transfer gene-regulatory information from somatic cells to germ cells may be able to communicate changes in the soma from one generation to the next. In the worm Caenorhabditis elegans, expression of double-stranded RNA (dsRNA) in neurons can result in the export of dsRNA-derived mobile RNAs to other distant cells. Here, we show that neuronal mobile RNAs can cause transgenerational silencing of a gene of matching sequence in germ cells. Consistent with neuronal mobile RNAs being forms of dsRNA, silencing of target genes that are expressed either in somatic cells or in the germline requires the dsRNA-selective importer SID-1. In contrast to silencing in somatic cells, which requires dsRNA expression in each generation, silencing in the germline is heritable after a single generation of exposure to neuronal mobile RNAs. Although initiation of inherited silencing within the germline requires SID-1, a primary Argonaute RDE-1, a secondary Argonaute HRDE-1, and an RNase D homolog MUT-7, maintenance of inherited silencing is independent of SID-1 and RDE-1, but requires HRDE-1 and MUT-7. Inherited silencing can persist for >25 generations in the absence of the ancestral source of neuronal dsRNA. Therefore, our results suggest that sequence-specific regulatory information in the form of dsRNA can be transferred from neurons to the germline to cause transgenerational silencing.
巻・号 112(7)
ページ 2133-8
公開日 2015-2-17
DOI 10.1073/pnas.1423333112
PII 1423333112
PMID 25646479
PMC PMC4343102
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Gene Silencing* Germ Cells* Neurons / metabolism* RNA, Double-Stranded / genetics*
IF 9.412
引用数 58
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm1200