RRC ID |
37460
|
著者 |
Shiraishi H.
|
タイトル |
Cryopreservation of the edible alkalophilic cyanobacterium Arthrospira platensis.
|
ジャーナル |
Biosci Biotechnol Biochem
|
Abstract |
Efficient cryopreservation conditions for the edible alkalophilic cyanobacterium Arthrospira (Spirulina) platensis were investigated using a model strain A. platensis NIES-39. As a result, it was found that more than 60% of cells were viable upon thawing, when they had been frozen at a cooling rate of approximately -1 °C min(-1) in the presence of 10% (v/v) dimethyl sulfoxide. Further examination with other Arthrospira strains showed that many of them had strain-dependent optimal conditions for cryopreservation. For example, the best freezing conditions for A. platensis SAG 21.99 were snap-freezing in liquid nitrogen in the presence of 5% (v/v) dimethyl sulfoxide, while they were slow cooling at approximately -1 °C min(-1) in the presence of 10% (v/v) methanol for A. platensis NIES-46, NIES-2308 and UTEX 1926. The variety of successful cryopreservation conditions presented in this study is useful when attempting to cryopreserve various Arthrospira strains.
|
巻・号 |
80(10)
|
ページ |
2051-7
|
公開日 |
2016-10-1
|
DOI |
10.1080/09168451.2016.1189320
|
PMID |
27240586
|
MeSH |
Cell Proliferation
Cell Survival
Cryopreservation / methods*
Species Specificity
Spirulina / cytology*
|
IF |
1.516
|
引用数 |
5
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
|
リソース情報 |
藻類 |
NIES-39
NIES-46
NIES-2308 |