RRC ID |
36770
|
著者 |
Kim YI, Mohri S, Hirai S, Lin S, Goto T, Ohyane C, Sakamoto T, Takahashi H, Shibata D, Takahashi N, Kawada T.
|
タイトル |
Tomato extract suppresses the production of proinflammatory mediators induced by interaction between adipocytes and macrophages.
|
ジャーナル |
Biosci Biotechnol Biochem
|
Abstract |
Obese adipose tissue is characterized by enhanced macrophage infiltration. A loop involving monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNFα) between adipocytes and macrophages establishes a vicious cycle that augments inflammatory changes and insulin resistance in obese adipose tissue. Tomatoes, one of the most popular crops worldwide, contain many beneficial phytochemicals that improve obesity-related diseases such as diabetes. Some of them have also been reported to have anti-inflammatory properties. In this study, we focused on the potential protective effects of phytochemicals in tomatoes on inflammation. We screened fractions of tomato extract using nitric oxide (NO) assay in lipopolysaccharide (LPS)-stimulated RAW264 macrophages. One fraction, RF52, significantly inhibited NO production in LPS-stimulated RAW264 macrophages. Furthermore, RF52 significantly decreased MCP-1 and TNFα productions. The coculture of 3T3-L1 adipocytes and RAW264 macrophages markedly enhanced MCP-1, TNFα, and NO productions compared with the control cultures; however, the treatment with RF52 inhibited the production of these proinflammatory mediators. These results suggest that RF52 from tomatoes may have the potential to suppress inflammation by inhibiting the production of NO or proinflammatory cytokines during the interaction between adipocytes and macrophages.
|
巻・号 |
79(1)
|
ページ |
82-7
|
公開日 |
2015-1-1
|
DOI |
10.1080/09168451.2014.962472
|
PMID |
25603813
|
MeSH |
3T3-L1 Cells
Adipocytes / cytology
Adipocytes / drug effects*
Adipocytes / metabolism
Animals
Anti-Inflammatory Agents / chemistry
Anti-Inflammatory Agents / pharmacology*
Cell Communication
Cell Differentiation
Cell Line
Chemokine CCL2 / antagonists & inhibitors
Chemokine CCL2 / biosynthesis
Coculture Techniques
Lipopolysaccharides / antagonists & inhibitors
Lipopolysaccharides / pharmacology
Macrophage Activation
Macrophages / cytology
Macrophages / drug effects*
Macrophages / metabolism
Mice
Nitric Oxide / antagonists & inhibitors
Nitric Oxide / biosynthesis
Phytochemicals / pharmacology*
Plant Extracts / chemistry
Plant Extracts / pharmacology*
Solanum lycopersicum / chemistry*
Tumor Necrosis Factor-alpha / antagonists & inhibitors
Tumor Necrosis Factor-alpha / biosynthesis
|
IF |
1.516
|
引用数 |
6
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CHEMISTRY, APPLIED
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
RAW 264(RCB0535) |