RRC ID |
30091
|
著者 |
Lloyd RG, Benson FE, Shurvinton CE.
|
タイトル |
Effect of ruv mutations on recombination and DNA repair in Escherichia coli K12.
|
ジャーナル |
Mol Gen Genet
|
Abstract |
Mutation of the ruv gene of E. coli is associated with sensitivity to radiation, and filamentous growth after transient inhibition of DNA synthesis. The filamentation of ruv strains is abolished by mutations in sfiA or sfiB that prevent SOS induced inhibition of cell division, but this does not restore resistance to UV radiation. Double mutants carrying both ruv and uvr mutations are considerably more sensitive to UV radiation than the single mutants, but there is no additive effect of ruv with recA, recF, recB, or recC mutations. ruv mutations have little effect on conjugal recombination in wild-type strains but confer recombination deficiency and extreme sensitivity to ionizing radiation in recBC sbcB strains. These results, together with the fact that ruv strains are excision proficient and mutable by UV light, are interpreted to suggest that the ruv + product is involved in recombinational repair of damaged DNA rather than in cell division as suggested by Otsuji et al. (1974).
|
巻・号 |
194(1-2)
|
ページ |
303-9
|
公開日 |
1984-1-1
|
DOI |
10.1007/BF00383532
|
PMID |
6374379
|
MeSH |
Bacterial Proteins / metabolism
DNA Ligases / metabolism
DNA Repair*
DNA, Bacterial / metabolism
DNA, Bacterial / radiation effects
Escherichia coli / genetics*
Escherichia coli / metabolism
Gene Expression Regulation*
Genes, Bacterial*
Recombination, Genetic*
|
引用数 |
165
|
WOS 分野
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
原核生物(大腸菌) |
ME8578
ME8579
ME8580
ME8581
ME8577 |