RRC ID |
19097
|
著者 |
Thong-On A, Suzuki K, Noda S, Inoue J, Kajiwara S, Ohkuma M.
|
タイトル |
Isolation and characterization of anaerobic bacteria for symbiotic recycling of uric acid nitrogen in the gut of various termites.
|
ジャーナル |
Microbes Environ
|
Abstract |
Recycling of the nitrogenous waste uric acid (UA) of wood-feeding termites by their gut bacteria is one of the significant aspects of symbiosis for the conservation of nitrogen sources. Diverse anaerobic UA-degrading bacteria comprising 16 species were isolated from the gut of eight termite species, and were assigned to Clostridia, Enterobacteriaceae, and low G+C Gram-positive cocci. UA-degrading Clostridia had never been isolated from termite guts. UA-degrading ability was sporadically distributed among phylogenetically various culturable anaerobic bacteria from termite guts. A strain of Clostridium sp., which was commonly isolated from three termite species and represented a probable new species in cluster XIVa of clostridia, utilized UA as a nitrogen source but not as a sole carbon and energy source. This feature is in clear contrast to that of well-studied purinolytic clostridia or previously isolated UA degraders from termite guts, which also utilize UA as a sole carbon and energy source. Ammonia is the major nitrogenous product of UA degradation. Various purines stimulated the growth of this strain when added to an otherwise growth-limiting, nitrogen poor medium. The bacterial species involved the recycling of UA nitrogen in the gut microbial community of termites are more diverse in terms of both taxonomy and nutritional physiology than previously recognized.
|
巻・号 |
27(2)
|
ページ |
186-92
|
公開日 |
2012-1-1
|
DOI |
10.1264/jsme2.me11325
|
PII |
DN/JST.JSTAGE/jsme2/ME11325
|
PMID |
22791052
|
PMC |
PMC4036019
|
MeSH |
Ammonia / metabolism
Animals
Bacteria, Anaerobic / classification
Bacteria, Anaerobic / isolation & purification*
Bacteria, Anaerobic / metabolism
Bacteria, Anaerobic / physiology*
Biodiversity*
Cluster Analysis
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Gastrointestinal Tract / microbiology
Isoptera / microbiology*
Molecular Sequence Data
Nitrogen / metabolism*
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Symbiosis*
Uric Acid / metabolism*
|
IF |
2.575
|
引用数 |
30
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 10513
JCM 10514
JCM 10515
JCM 10519
JCM 10522
JCM 10521
JCM 10523
JCM 10537
JCM 1297
JCM 1415
JCM 17987
JCM 17988
JCM 17989
JCM 17990
JCM 17991
JCM 17992
JCM 17993
JCM 17994 |