RRC ID |
27314
|
著者 |
Koike S, Matsukura C, Takayama M, Asamizu E, Ezura H.
|
タイトル |
Suppression of γ-aminobutyric acid (GABA) transaminases induces prominent GABA accumulation, dwarfism and infertility in the tomato (Solanum lycopersicum L.).
|
ジャーナル |
Plant Cell Physiol
|
Abstract |
Tomatoes accumulate γ-aminobutyric acid (GABA) at high levels in the immature fruits. GABA is rapidly converted to succinate during fruit ripening through the activities of GABA transaminase (GABA-T) and succinate semialdehyde dehydrogenase (SSADH). Although three genes encoding GABA-T and both pyruvate- and α-ketoglutarate-dependent GABA-T activities have been detected in tomato fruits, the mechanism underlying the GABA-T-mediated conversion of GABA has not been fully understood. In this work, we conducted loss-of-function analyses utilizing RNA interference (RNAi) transgenic plants with suppressed pyruvate- and glyoxylate-dependent GABA-T gene expression to clarify which GABA-T isoforms are essential for its function. The RNAi plants with suppressed SlGABA-T gene expression, particularly SlGABA-T1, showed severe dwarfism and infertility. SlGABA-T1 expression was inversely associated with GABA levels in the fruit at the red ripe stage. The GABA contents in 35S::SlGABA-T1(RNAi) lines were 1.3-2.0 times and 6.8-9.2 times higher in mature green and red ripe fruits, respectively, than the contents in wild-type fruits. In addition, SlGABA-T1 expression was strongly suppressed in the GABA-accumulating lines. These results indicate that pyruvate- and glyoxylate-dependent GABA-T is the essential isoform for GABA metabolism in tomato plants and that GABA-T1 primarily contributes to GABA reduction in the ripening fruits.
|
巻・号 |
54(5)
|
ページ |
793-807
|
公開日 |
2013-5-1
|
DOI |
10.1093/pcp/pct035
|
PII |
pct035
|
PMID |
23435575
|
MeSH |
4-Aminobutyrate Transaminase / metabolism*
Amino Acids / metabolism
Flowers / growth & development
Fruit / growth & development
Gene Expression Regulation, Plant
Genes, Plant / genetics
Glutamic Acid / metabolism
Lycopersicon esculentum / enzymology*
Lycopersicon esculentum / genetics
Lycopersicon esculentum / growth & development*
Metabolic Networks and Pathways / genetics
Plant Infertility*
Plant Leaves / enzymology
Plant Leaves / genetics
Plant Stems / metabolism
Plants, Genetically Modified
RNA Interference
Suppression, Genetic*
gamma-Aminobutyric Acid / metabolism*
|
IF |
4.062
|
引用数 |
25
|
WOS 分野
|
PLANT SCIENCES
CELL BIOLOGY
|
リソース情報 |
トマト |
TOMJPF00001 |