RRC ID |
48517
|
著者 |
Higuchi-Takeuchi M, Morisaki K, Toyooka K, Numata K.
|
タイトル |
Synthesis of High-Molecular-Weight Polyhydroxyalkanoates by Marine Photosynthetic Purple Bacteria.
|
ジャーナル |
PLoS One
|
Abstract |
Polyhydroxyalkanoate (PHA) is a biopolyester/bioplastic that is produced by a variety of microorganisms to store carbon and increase reducing redox potential. Photosynthetic bacteria convert carbon dioxide into organic compounds using light energy and are known to accumulate PHA. We analyzed PHAs synthesized by 3 purple sulfur bacteria and 9 purple non-sulfur bacteria strains. These 12 purple bacteria were cultured in nitrogen-limited medium containing acetate and/or sodium bicarbonate as carbon sources. PHA production in the purple sulfur bacteria was induced by nitrogen-limited conditions. Purple non-sulfur bacteria accumulated PHA even under normal growth conditions, and PHA production in 3 strains was enhanced by nitrogen-limited conditions. Gel permeation chromatography analysis revealed that 5 photosynthetic purple bacteria synthesized high-molecular-weight PHAs, which are useful for industrial applications. Quantitative reverse transcription polymerase chain reaction analysis revealed that mRNA levels of phaC and PhaZ genes were low under nitrogen-limited conditions, resulting in production of high-molecular-weight PHAs. We conclude that all 12 tested strains are able to synthesize PHA to some degree, and we identify 5 photosynthetic purple bacteria that accumulate high-molecular-weight PHA molecules. Furthermore, the photosynthetic purple bacteria synthesized PHA when they were cultured in seawater supplemented with acetate. The photosynthetic purple bacteria strains characterized in this study should be useful as host microorganisms for large-scale PHA production utilizing abundant marine resources and carbon dioxide.
|
巻・号 |
11(8)
|
ページ |
e0160981
|
公開日 |
2016-1-1
|
DOI |
10.1371/journal.pone.0160981
|
PII |
PONE-D-16-26334
|
PMID |
27513570
|
PMC |
PMC4981452
|
MeSH |
Bacterial Proteins / metabolism*
Molecular Weight
Photosynthesis*
Polyhydroxyalkanoates / biosynthesis*
Proteobacteria / metabolism*
|
IF |
2.74
|
引用数 |
27
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 14796
JCM 14262
JCM 17835
JCM 13911
JCM 13533
JCM 14780
JCM 14889
JCM 14149
JCM 14150
JCM 14544
JCM 13532
JCM 9337
JCM 13300
JCM 13589
JCM 13531 |