RRC ID |
48845
|
著者 |
Fukumori H, Lee J, Fujii T, Kajiura Z, Banno Y.
|
タイトル |
Long-term preservation of eri and ailanthus silkworms using frozen gonads.
|
ジャーナル |
Cryobiology
|
Abstract |
Cryopreservation of eri and ailanthus silkworms using frozen gonads was investigated. First, we evaluated the freeze tolerance of ovary and testis in the eri silkworm, which showed high tolerance. Mating between frozen ovary-transplanted females and frozen testis-transplanted males produced 163.0 eggs, yielding 105.7 larvae per moth. In a second experiment, we tested the use of the eri silkworm as a host insect for gonad transplantation from ailanthus silkworm donors. A high success ratio for laid and hatched eggs was demonstrated for ovary transplantation (97.8 and 51.3 eggs per moth, respectively). For testis transplantation, however, the average number of hatched larvae was low (12.0). Mating between host eri females and males in which both frozen ovary and testis of the ailanthus silkworm had been transplanted produced 6.4 fertilized eggs per host moth. Our success in using cross subspecies cryopreservation between these wild silkworms could lead to the alternative use of hosts between species in other insects.
|
巻・号 |
77
|
ページ |
71-74
|
公開日 |
2017-8-1
|
DOI |
10.1016/j.cryobiol.2017.05.003
|
PII |
S0011-2240(17)30099-8
|
PMID |
28502526
|
MeSH |
Animals
Bombyx*
Cryopreservation*
Female
Freezing
Larva
Male
Organ Transplantation
Ovary*
Reproduction
Testis*
|
IF |
2.287
|
引用数 |
1
|
リソース情報 |
カイコ |
Eri silkworm
Ailanthus silkworm |