論文 - 詳細
RRC ID | 36993 |
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著者 | Yokoo T, Matsumoto K, Ooba T, Morimoto K, Taguchi S. |
タイトル | Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase. |
ジャーナル | Biosci Biotechnol Biochem |
Abstract |
Highly active mutant of NADPH-dependent acetoacetyl-CoA reductase (PhaB) was expressed in Nicotiana tabacum cv. Bright Yellow-2 cultured cells to produce poly(3-hydroxybutyrate) [P(3HB)]. The mutated PhaB increased P(3HB) content by three-fold over the control, indicating that the mutant was a versatile tool for P(3HB) production. Additionally, the PhaB-catalyzed reaction was suggested to be a rate-limiting step of P(3HB) biosynthesis in tobacco BY-2 cells. |
巻・号 | 79(6) |
ページ | 986-8 |
公開日 | 2015-1-1 |
DOI | 10.1080/09168451.2014.1002448 |
PMID | 25647430 |
MeSH | Alcohol Oxidoreductases / genetics* Alcohol Oxidoreductases / metabolism Biocatalysis Cell Line Hydroxybutyrates / metabolism* Kinetics Plants, Genetically Modified Polyesters / metabolism* Protein Engineering* Tobacco / cytology* Tobacco / genetics* Transformation, Genetic |
IF | 1.516 |
引用数 | 3 |
WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CHEMISTRY, APPLIED |
リソース情報 | |
シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00001 |