| RRC ID |
32636
|
| 著者 |
Hashiguchi M, Kashiwakura Y, Kojima H, Kobayashi A, Kanno Y, Kobata T.
|
| タイトル |
IL-33 activates eosinophils of visceral adipose tissue both directly and via innate lymphoid cells.
|
| ジャーナル |
Eur J Immunol
|
| Abstract |
Eosinophils are multifunctional leukocytes involved in allergic reactions as well as adipose tissue regulation. IL-5 is required for eosinophil survival; however, the in vivo mechanisms of eosinophil regulation are not fully understood. A tg mouse model with il5 promoter-driven EGFP expression was established for detecting the IL-5-producing cells in vivo. Il5-egfp tg mice expressed high levels of EGFP in gonadal adipose tissue (GAT) cells. EGFP(+) cells in GAT were mainly group 2 innate lymphoid cells (ILCs). IL-33 preferentially expanded EGFP(+) cells and eosinophils in GAT in vivo. EGFP(+) ILCs were found to upregulate prg2 mRNA expression in GAT eosinophils. These results demonstrate that ILCs activate eosinophils in GAT. The blockage of IL-33Rα, on the other hand, did not impair EGFP(+) ILC numbers but did impair eosinophil numbers in vivo. GAT eosinophils expressed IL-33Rα and IL-33 expanded eosinophil numbers in CD90(+) cell-depleted mice. IL-33 was further observed to induce the expression of retnla and epx mRNA in eosinophils. These findings demonstrate that IL-33 directly activates eosinophils in GAT, and together with our other findings described above, our findings show that IL-33 has dual pathways via which it activates eosinophils in vivo: a direct activation pathway and a group 2 ILC-mediated pathway.
|
| 巻・号 |
45(3)
|
| ページ |
876-85
|
| 公開日 |
2015-3-1
|
| DOI |
10.1002/eji.201444969
|
| PMID |
25504587
|
| MeSH |
Animals
Eosinophil Major Basic Protein / genetics
Eosinophil Major Basic Protein / immunology
Eosinophils / cytology
Eosinophils / immunology*
Gene Expression Regulation / immunology*
Immunity, Innate / physiology*
Intercellular Signaling Peptides and Proteins / genetics
Intercellular Signaling Peptides and Proteins / immunology
Interleukin-33
Interleukin-5 / genetics
Interleukin-5 / immunology
Interleukins / genetics
Interleukins / immunology*
Intra-Abdominal Fat / cytology
Intra-Abdominal Fat / immunology*
Mice
Mice, Knockout
|
| IF |
4.404
|
| 引用数 |
27
|
|
WOS 分野
|
IMMUNOLOGY
|
| リソース情報 |
| 遺伝子材料 |
B6N BAC Mouse (RDB07573) B6Ng01–038M13 |
