| RRC ID |
83968
|
| 著者 |
Nikonorova IA, desRanleau E, Jacobs KC, Saul J, Walsh JD, Wang J, Barr MM.
|
| タイトル |
Polycystins recruit cargo to distinct ciliary extracellular vesicle subtypes.
|
| ジャーナル |
bioRxiv
|
| Abstract |
Therapeutic use of tiny extracellular vesicles (EVs) requires understanding cargo loading mechanisms. Here, we used a modular proximity label approach to identify EV cargo associated with the transient potential channel (TRP) polycystin PKD-2 of C. elegans. Polycystins are conserved receptor-TRP channel proteins affecting cilium function; dysfunction causes polycystic kidney disease in humans and mating deficits in C. elegans. Polycystin-2 EV localization is conserved from algae to humans, hinting at an ancient and unknown function. We discovered that polycystins associate with and direct specific cargo to EVs: channel-like PACL-1, dorsal and ventral membrane C-type lectins PAMLs, and conserved tumor necrosis-associated factor (TRAF) signaling adaptors TRF-1 and TRF-2. Loading of these components relied on polycystin-1 LOV-1. Our modular EV-TurboID approach can be applied in both cell- and tissue-specific manners to define the composition of distinct EV subtypes, addressing a major challenge of the EV field.
|
| 公開日 |
2024-4-18
|
| DOI |
10.1101/2024.04.17.588758
|
| PII |
2024.04.17.588758
|
| PMID |
38659811
|
| PMC |
PMC11042387
|
| リソース情報 |
| 線虫 |
tm5167
tm12580
tm12527 |
