| RRC ID |
88110
|
| 著者 |
Nikonorova IA, desRanleau E, Jacobs KC, Saul J, Walsh JD, Wang J, Barr MM.
|
| タイトル |
Polycystins recruit cargo to distinct ciliary extracellular vesicle subtypes in C. elegans.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Therapeutic use of tiny extracellular vesicles (EVs) requires understanding cargo loading mechanisms. Here, we use a modular proximity labeling approach to identify the cargo of ciliary EVs associated with the transient receptor potential channel polycystin-2 PKD-2 of C. elegans. Polycystins are conserved ciliary proteins and cargo of EVs; dysfunction causes polycystic kidney disease in humans and mating deficits in C. elegans. We discover that polycystins localize with specific cargo on ciliary EVs: polycystin-associated channel-like protein PACL-1, dorsal and ventral polycystin-associated membrane C-type lectins PAMLs, and conserved tumor necrosis factor receptor-associated factor (TRAF) TRF-1 and TRF-2. Loading of these components to EVs relies on polycystin-1 LOV-1. Our modular EV-TurboID approach can be applied in both cell- and tissue-specific manners to define the composition of distinct EV subtypes, addressing a major challenge of the EV field.
|
| 巻・号 |
16(1)
|
| ページ |
2899
|
| 公開日 |
2025-4-3
|
| DOI |
10.1038/s41467-025-57512-3
|
| PII |
10.1038/s41467-025-57512-3
|
| PMID |
40180912
|
| PMC |
PMC11968823
|
| MeSH |
Animals
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / metabolism
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
Cilia* / metabolism
Extracellular Vesicles* / metabolism
Humans
Lectins, C-Type / metabolism
TRPP Cation Channels* / genetics
TRPP Cation Channels* / metabolism
|
| リソース情報 |
| 線虫 |
tm12527
tm12580
tm5167 |
